Restriction map for
pF1KB8112
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 238 GTAGAC GT^[AC][GT]AC
AcsI 221 AAATTT [GA]^AATT[CT]
AfaI 205 GTAC GT^AC
AluI 417 AGCT AG^CT
AlwNI 56 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 124 CCTCAGG CC^T[ACGT]AGG
ApoI 221 AAATTT [GA]^AATT[CT]
Asp700I 52 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 338 GTGCTC G[AT]GC[AT]^C
AvaII 284 GGTCC G^G[AT]CC
BbsI 387 GAAGAC GAAGAC(2/6)
BcnI 5 CCGGG CC^[GC]GG
BmyI 338 GTGCTC G[AGT]GC[ACT]^C
BpuAI 387 GAAGAC GAAGAC(2/6)
BsiHKAI 338 GTGCTC G[AT]GC[AT]^C
Bsp1286I 338 GTGCTC G[AGT]GC[ACT]^C
BspDI 318 ATCGAT AT^CGAT
BspHI 212 TCATGA T^CATGA
BsrDI 114 CATTGC GCAATG(2/0)
BsrI 55 CCAGT ACTGG(1/-1)
BstF5I 229 CATCC GGATG(2/0)
BstYI 299 AGATCC [GA]^GATC[CT]
Bsu36I 124 CCTCAGG CC^T[ACGT]AGG
ClaI 318 ATCGAT AT^CGAT
DdeI 125 CTCAG C^T[ACGT]AG
DraI 154 TTTAAA TTT^AAA
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eco81I 124 CCTCAGG CC^T[ACGT]AGG
FauI 14 CCCGC CCCGC(4/6)
FokI 229 CATCC GGATG(9/13)
HapII 5 CCGG C^CGG
HinfI 130 GATTC G^A[ACGT]TC
HpaII 5 CCGG C^CGG
MaeII 207 ACGT A^CGT
MflI 299 AGATCC [GA]^GATC[CT]
MslI 229 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 5 CCGG C^CGG
NciI 5 CCGGG CC^[GC]GG
NlaIV 284 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 55 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 295 CTGCAG CTGCA^G
RcaI 212 TCATGA T^CATGA
RsaI 205 GTAC GT^AC
SfaNI 65 GCATC GCATC(5/9)
SfcI 295 CTGCAG C^T[GA][CT]AG
SspI 451 AATATT AAT^ATT
StuI 326 AGGCCT AGG^CCT
TaqI 319 TCGA T^CGA
TfiI 130 GATTC G^A[AT]TC
Tsp509I 222 AATT ^AATT
TthHB8I 319 TCGA T^CGA
Van91I 55 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 299 AGATCC [GA]^GATC[CT]
XmnI 52 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbvI 193 GCAGC GCAGC(8/12)
9 GCTGC
BfaI 419 CTAG C^TAG
431 CTAG
BsmFI 148 GGGAC GGGAC(10/14)
285 GTCCC
BstNI 61 CCTGG CC^[AT]GG
324 CCAGG
Cac8I 45 GCTGGC GC[ACGT]^[ACGT]GC
118 GCTGGC
Cfr13I 121 GGCCC G^G[ACGT]CC
284 GGTCC
EcoRII 61 CCTGG ^CC[AT]GG
324 CCAGG
Fnu4HI 9 GCTGC GC^[ACGT]GC
193 GCAGC
ItaI 9 GCTGC GC^[ACGT]GC
193 GCAGC
MaeI 419 CTAG C^TAG
431 CTAG
MseI 155 TTAA T^TAA
334 TTAA
MvaI 61 CCTGG CC^[AT]GG
324 CCAGG
Sau96I 121 GGCCC G^G[ACGT]CC
284 GGTCC
Tru9I 155 TTAA T^TAA
334 TTAA
TseI 9 GCTGC G^C[AT]GC
193 GCAGC
TspRI 293 CACTG CA[GC]TG[ACGT][ACGT]^
382 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI AlwI BsiYI BslI BssKI
DpnII HaeIII MboI MboII MnlI
NdeII NlaIII Sau3AI ScrFI