Restriction map for
pF1KB8107
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HphI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MroI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccII 285 CGCG CG^CG
AcsI 500 GAATTC [GA]^AATT[CT]
AlwI 413 GGATC GGATC(4/5)
ApoI 500 GAATTC [GA]^AATT[CT]
BclI 200 TGATCA T^GATCA
BcnI 644 CCCGG CC^[GC]GG
BsmFI 642 GTCCC GGGAC(10/14)
BstUI 285 CGCG CG^CG
BstXI 35 CCAAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 523 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 523 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 500 GAATTC G^AATTC
EcoRV 106 GATATC GAT^ATC
EcoT22I 233 ATGCAT ATGCA^T
FbaI 200 TGATCA T^GATCA
HapII 645 CCGG C^CGG
HincII 88 GTTGAC GT[CT]^[GA]AC
HindII 88 GTTGAC GT[CT]^[GA]AC
HpaII 645 CCGG C^CGG
MaeIII 550 GTGAC ^GT[ACGT]AC
MspI 645 CCGG C^CGG
MvnI 285 CGCG CG^CG
NciI 644 CCCGG CC^[GC]GG
NsiI 233 ATGCAT ATGCA^T
PstI 310 CTGCAG CTGCA^G
SapI 315 GAAGAGC GCTCTTC(1/4)
SfcI 310 CTGCAG C^T[GA][CT]AG
Tsp45I 550 GTGAC ^GT[GC]AC
TspRI 257 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AluI 227 AGCT AG^CT
281 AGCT
AspHI 259 GTGCTC G[AT]GC[AT]^C
318 GAGCAC
BalI 188 TGGCCA TGG^CCA
650 TGGCCA
BmyI 259 GTGCTC G[AGT]GC[ACT]^C
318 GAGCAC
BpmI 578 CTCCAG CTGGAG(16/14)
628 CTGGAG
BsiHKAI 259 GTGCTC G[AT]GC[AT]^C
318 GAGCAC
BsiYI 279 CCAGCTCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
374 CCACCATGAGG
BslI 279 CCAGCTCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
374 CCACCATGAGG
Bsp1286I 259 GTGCTC G[AGT]GC[ACT]^C
318 GAGCAC
BsrI 42 ACTGG ACTGG(1/-1)
542 CCAGT
BstNI 21 CCAGG CC^[AT]GG
580 CCAGG
Cfr13I 162 GGGCC G^G[ACGT]CC
524 GGGCC
DdeI 255 CTCAG C^T[ACGT]AG
597 CTCAG
EaeI 188 TGGCCA [CT]^GGCC[GA]
650 TGGCCA
EarI 315 GAAGAG CTCTTC(1/4)
619 GAAGAG
EcoRII 21 CCAGG ^CC[AT]GG
580 CCAGG
EcoT14I 191 CCAAGG C^C[AT][AT]GG
527 CCTTGG
HinfI 357 GATTC G^A[ACGT]TC
366 GATTC
Ksp632I 315 GAAGAG CTCTTC(1/4)
619 GAAGAG
MluNI 188 TGGCCA TGG^CCA
650 TGGCCA
MscI 188 TGGCCA TGG^CCA
650 TGGCCA
MslI 264 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
321 CACAGAAGTG
MvaI 21 CCAGG CC^[AT]GG
580 CCAGG
NlaIV 304 GGTTCC GG[ACGT]^[ACGT]CC
523 GGGGCC
Sau96I 162 GGGCC G^G[ACGT]CC
524 GGGCC
StyI 191 CCAAGG C^C[AT][AT]GG
527 CCTTGG
TfiI 357 GATTC G^A[AT]TC
366 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI BbvI BsaJI BseRI BssKI
BstF5I Cac8I DpnII Fnu4HI FokI
HaeIII ItaI MboI MboII MnlI
MwoI NdeII NlaIII Sau3AI ScrFI
SfaNI TseI Tsp509I