Restriction map for
pF1KB7789
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BciVI BclI
BfaI BfrI BglI BlnI BsaHI
BsaI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI MaeI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NruI NsiI NspI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 310 GTAGAC GT^[AC][GT]AC
AcsI 262 GAATTC [GA]^AATT[CT]
ApoI 262 GAATTC [GA]^AATT[CT]
AspI 1315 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 797 TGGCCA TGG^CCA
BanI 1335 GGCACC G^G[CT][GA]CC
BbrPI 1237 CACGTG CAC^GTG
BcgI 561 CGAAATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 716 AGATCT A^GATCT
BlpI 1074 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1074 GCTCAGC GC^T[ACGT]AGC
BsaBI 543 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 195 ACCGGA [AT]^CCGG[AT]
BseRI 1267 GAGGAG GAGGAG(10/8)
BsgI 454 GTGCAG GTGCAG(16/14)
BsmBI 120 CGTCTC CGTCTC(1/5)
BssHII 139 GCGCGC G^CGCGC
CelII 1074 GCTCAGC GC^T[ACGT]AGC
DraIII 274 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 975 CCGCGG C^C[GA][CT]GG
EagI 86 CGGCCG C^GGCCG
EarI 660 GAAGAG CTCTTC(1/4)
EclXI 86 CGGCCG C^GGCCG
Eco52I 86 CGGCCG C^GGCCG
EcoNI 861 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 262 GAATTC G^AATTC
HaeII 327 GGCGCT [GA]GCGC^[CT]
HgaI 119 GCGTC GACGC(5/10)
HindIII 802 AAGCTT A^AGCTT
Ksp632I 660 GAAGAG CTCTTC(1/4)
KspI 975 CCGCGG CCGC^GG
MamI 543 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 797 TGGCCA TGG^CCA
MscI 797 TGGCCA TGG^CCA
MseI 287 TTAA T^TAA
NaeI 188 GCCGGC GCC^GGC
NgoMIV 188 GCCGGC G^CCGGC
NotI 85 GCGGCCGC GC^GGCCGC
PaeR7I 233 CTCGAG C^TCGAG
PflFI 1315 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 614 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1237 CACGTG CAC^GTG
PmlI 1237 CACGTG CAC^GTG
PpuMI 1148 AGGACCT [GA]G^G[AT]CC[CT]
SacII 975 CCGCGG CCGC^GG
SapI 660 GAAGAGC GCTCTTC(1/4)
TaqI 234 TCGA T^CGA
Tru9I 287 TTAA T^TAA
Tth111I 1315 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 234 TCGA T^CGA
Van91I 614 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 233 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 781 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1086 CAGCTACTG
ApaI 70 GGGCCC GGGCC^C
884 GGGCCC
BbsI 459 GAAGAC GAAGAC(2/6)
595 GAAGAC
BbvCI 1152 CCTCAGC CCTCAGC(-5/-2)
91 GCTGAGG
BpmI 1024 CTGGAG CTGGAG(16/14)
1108 CTGGAG
BpuAI 459 GAAGAC GAAGAC(2/6)
595 GAAGAC
BsaAI 277 TACGTG [CT]AC^GT[GA]
1237 CACGTG
BsiEI 86 CGGCCG CG[GA][CT]^CG
193 CGACCG
BsmAI 121 GTCTC GTCTC(1/5)
231 GTCTC
BsmI 81 GAATGC GAATGC(1/-1)
210 GCATTC
Bsp1407I 270 TGTACA T^GTACA
354 TGTACA
BsrGI 270 TGTACA T^GTACA
354 TGTACA
DraII 883 GGGGCCC [GA]G^G[ACGT]CC[CT]
1148 AGGACCT
EaeI 86 CGGCCG [CT]^GGCC[GA]
797 TGGCCA
EcoO109I 883 GGGGCCC [GA]G^G[ACGT]CC[CT]
1148 AGGACCT
HinfI 919 GAGTC G^A[ACGT]TC
1315 GACTC
MaeIII 157 GTCAC ^GT[ACGT]AC
1249 GTCAC
PleI 1315 GACTC GAGTC(4/5)
919 GAGTC
SacI 385 GAGCTC GAGCT^C
1012 GAGCTC
ScaI 632 AGTACT AGT^ACT
1220 AGTACT
SspBI 270 TGTACA T^GTACA
354 TGTACA
Tsp45I 157 GTCAC ^GT[GC]AC
1249 GTCAC
Tsp509I 263 AATT ^AATT
564 AATT
XcmI 485 CCACAGCAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
800 CCAAGCTTTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI StyI TseI TspRI
XhoII XmaCI XmaI