Restriction map for
pF1KB7703
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# NO CUT ENZYMES ( 124 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI ApoI AscI AseI
AsnI AspI AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HgaI HindIII
HpaI KasI MaeI MaeIII MamI
MfeI MluI MroI MseI MunI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 394 GACGTC GACGT^C
Acc65I 798 GGTACC G^GTACC
AcyI 394 GACGTC G[GA]^CG[CT]C
AhdI 1096 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1204 GTGCAC G^TGCAC
AocI 478 CCTGAGG CC^T[ACGT]AGG
Aor51HI 569 AGCGCT AGC^GCT
ApaLI 1204 GTGCAC G^TGCAC
Asp718I 798 GGTACC G^GTACC
AspEI 1096 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1204 GTGCAC G[AT]GC[AT]^C
AvaI 367 CCCGAG C^[CT]CG[GA]G
AviII 836 TGCGCA TGC^GCA
BalI 1063 TGGCCA TGG^CCA
BsaHI 394 GACGTC G[GA]^CG[CT]C
BsaI 904 GGTCTC GGTCTC(1/5)
BsiHKAI 1204 GTGCAC G[AT]GC[AT]^C
BsmBI 114 CGTCTC CGTCTC(1/5)
BsoBI 367 CCCGAG C^[CT]CG[GA]G
Bsp1407I 992 TGTACA T^GTACA
BsrBI 1227 CCGCTC CCGCTC(-3/-3)
BsrGI 992 TGTACA T^GTACA
BssSI 784 CTCGTG CACGAG(-5/-1)
BstAPI 601 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 665 AGATCC [GA]^GATC[CT]
Bsu36I 478 CCTGAGG CC^T[ACGT]AGG
DraIII 584 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1096 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 433 GAAGAG CTCTTC(1/4)
Eco47III 569 AGCGCT AGC^GCT
Eco81I 478 CCTGAGG CC^T[ACGT]AGG
FspI 836 TGCGCA TGC^GCA
Hin1I 394 GACGTC G[GA]^CG[CT]C
HincII 757 GTCAAC GT[CT]^[GA]AC
HindII 757 GTCAAC GT[CT]^[GA]AC
KpnI 798 GGTACC GGTAC^C
Ksp632I 433 GAAGAG CTCTTC(1/4)
KspI 1216 CCGCGG CCGC^GG
MaeII 395 ACGT A^CGT
MflI 665 AGATCC [GA]^GATC[CT]
MluNI 1063 TGGCCA TGG^CCA
MscI 1063 TGGCCA TGG^CCA
NspI 98 ACATGC [GA]CATG^[CT]
PvuII 521 CAGCTG CAG^CTG
SacII 1216 CCGCGG CCGC^GG
SexAI 585 ACCAGGT A^CC[AT]GGT
SspBI 992 TGTACA T^GTACA
StuI 198 AGGCCT AGG^CCT
TfiI 691 GAATC G^A[AT]TC
XhoII 665 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BsgI 1056 CTGCAC GTGCAG(16/14)
599 GTGCAG
BsmAI 115 GTCTC GTCTC(1/5)
905 GTCTC
BspMI 818 ACCTGC ACCTGC(4/8)
1022 ACCTGC
BsrDI 840 CATTGC GCAATG(2/0)
855 CATTGC
EaeI 81 TGGCCG [CT]^GGCC[GA]
1063 TGGCCA
EcoNI 320 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
580 CCTCCACCAGG
HaeII 569 AGCGCT [GA]GCGC^[CT]
1115 GGCGCT
NaeI 83 GCCGGC GCC^GGC
1028 GCCGGC
NgoMIV 83 GCCGGC G^CCGGC
1028 GCCGGC
PflMI 583 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
747 CCAGCTCCTGG
PleI 166 GACTC GAGTC(4/5)
464 GAGTC
PpuMI 473 AGGTCCC [GA]G^G[AT]CC[CT]
879 GGGACCC
SmlI 170 CTCAAG C^T[CT][GA]AG
1012 CTCAAG
Van91I 583 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
747 CCAGCTCCTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI XcmI XmnI