##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
BalI 410 TGGCCA TGG^CCA
BamHI 403 GGATCC G^GATCC
BanI 648 GGTGCC G^G[CT][GA]CC
BanII 601 GGGCTC G[GA]GC[CT]^C
BbsI 502 GAAGAC GAAGAC(2/6)
BbvCI 314 CCTCAGC CCTCAGC(-5/-2)
BmyI 601 GGGCTC G[AGT]GC[ACT]^C
BpmI 881 CTCCAG CTGGAG(16/14)
BpuAI 502 GAAGAC GAAGAC(2/6)
BsaAI 849 TACGTG [CT]AC^GT[GA]
BsaBI 700 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 248 GAGACC GGTCTC(1/5)
BseRI 604 CTCCTC GAGGAG(10/8)
Bsp1286I 601 GGGCTC G[AGT]GC[ACT]^C
BstAPI 672 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 55 CCAATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 865 TTTAAA TTT^AAA
EaeI 410 TGGCCA [CT]^GGCC[GA]
EcoNI 606 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 108 ATGCAT ATGCA^T
HaeIII 411 GGCC GG^CC
HphI 486 GGTGA GGTGA(8/7)
MaeII 850 ACGT A^CGT
MamI 700 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 410 TGGCCA TGG^CCA
MscI 410 TGGCCA TGG^CCA
NsiI 108 ATGCAT ATGCA^T
TaqI 774 TCGA T^CGA
TthHB8I 774 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 639 GTAC GT^AC
845 GTAC
AlwI 404 GATCC GGATC(4/5)
403 GGATC
BglII 348 AGATCT A^GATCT
712 AGATCT
BsaJI 252 CCCTGG C^C[ACGT][ACGT]GG
407 CCCTGG
BsiYI 593 CCGACTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
606 CCTCCAACAGG
BslI 593 CCGACTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
606 CCTCCAACAGG
BspHI 149 TCATGA T^CATGA
530 TCATGA
BsrI 736 CCAGT ACTGG(1/-1)
883 CCAGT
EarI 665 CTCTTC CTCTTC(1/4)
337 GAAGAG
HincII 377 GTCAAC GT[CT]^[GA]AC
745 GTTGAC
HindII 377 GTCAAC GT[CT]^[GA]AC
745 GTTGAC
Ksp632I 665 CTCTTC CTCTTC(1/4)
337 GAAGAG
PleI 589 GACTC GAGTC(4/5)
595 GACTC
PstI 497 CTGCAG CTGCA^G
670 CTGCAG
RcaI 149 TCATGA T^CATGA
530 TCATGA
RsaI 639 GTAC GT^AC
845 GTAC
SspI 562 AATATT AAT^ATT
819 AATATT
TspRI 507 CAGTG CA[GC]TG[ACGT][ACGT]^
884 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI ApoI BbvI BsmAI BssKI
BstNI BstYI Cac8I DdeI DpnII
EcoRII Fnu4HI HinfI ItaI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I XhoII