##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DpnII DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HindIII HpaI KpnI MamI
MboI MfeI MflI MluI MroI
MseI MslI MunI NaeI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI Sau3AI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I XbaI XcmI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 402 GGCGCC G[GA]^CG[CT]C
Alw44I 844 GTGCAC G^TGCAC
ApaLI 844 GTGCAC G^TGCAC
AscI 338 GGCGCGCC GG^CGCGCC
BalI 791 TGGCCA TGG^CCA
BbeI 402 GGCGCC GGCGC^C
BbsI 559 GTCTTC GAAGAC(2/6)
BcgI 276 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 229 CTAG C^TAG
BglI 733 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 15 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 15 GCTCAGC GC^T[ACGT]AGC
BpuAI 559 GTCTTC GAAGAC(2/6)
BsaHI 402 GGCGCC G[GA]^CG[CT]C
BsiWI 455 CGTACG C^GTACG
BsmFI 770 GGGAC GGGAC(10/14)
BsrFI 335 ACCGGC [GA]^CCGG[CT]
BssHII 339 GCGCGC G^CGCGC
CelII 15 GCTCAGC GC^T[ACGT]AGC
Cfr10I 335 ACCGGC [GA]^CCGG[CT]
EarI 750 CTCTTC CTCTTC(1/4)
HaeII 402 GGCGCC [GA]GCGC^[CT]
HgaI 58 GACGC GACGC(5/10)
Hin1I 402 GGCGCC G[GA]^CG[CT]C
HincII 529 GTCAAC GT[CT]^[GA]AC
HindII 529 GTCAAC GT[CT]^[GA]AC
HinfI 118 GACTC G^A[ACGT]TC
KasI 402 GGCGCC G^GCGCC
Ksp632I 750 CTCTTC CTCTTC(1/4)
MaeI 229 CTAG C^TAG
MaeIII 779 GTAAC ^GT[ACGT]AC
MluNI 791 TGGCCA TGG^CCA
MscI 791 TGGCCA TGG^CCA
NarI 402 GGCGCC GG^CGCC
NcoI 590 CCATGG C^CATGG
PaeR7I 783 CTCGAG C^TCGAG
PflMI 628 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 118 GACTC GAGTC(4/5)
PpuMI 770 GGGACCC [GA]G^G[AT]CC[CT]
PstI 643 CTGCAG CTGCA^G
SbfI 642 CCTGCAGG CCTGCA^GG
SfoI 402 GGCGCC GGC^GCC
SmlI 783 CTCGAG C^T[CT][GA]AG
Sse8387I 642 CCTGCAGG CCTGCA^GG
StuI 128 AGGCCT AGG^CCT
TaqI 784 TCGA T^CGA
TthHB8I 784 TCGA T^CGA
Van91I 628 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 783 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 638 GGGCCC GGGCC^C
660 GGGCCC
AvaII 293 GGACC G^G[AT]CC
771 GGACC
BseRI 277 GAGGAG GAGGAG(10/8)
433 GAGGAG
BsiEI 680 CGGCCG CG[GA][CT]^CG
722 CGGCCG
BsrBI 406 CCGCTC CCGCTC(-3/-3)
490 CCGCTC
BsrI 464 ACTGG ACTGG(1/-1)
882 CCAGT
EagI 680 CGGCCG C^GGCCG
722 CGGCCG
EclXI 680 CGGCCG C^GGCCG
722 CGGCCG
Eco52I 680 CGGCCG C^GGCCG
722 CGGCCG
EcoNI 239 CCTTCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
300 CCTCACCGAGG
EcoT14I 473 CCAAGG C^C[AT][AT]GG
590 CCATGG
HphI 206 TCACC GGTGA(8/7)
302 TCACC
KspI 605 CCGCGG CCGC^GG
737 CCGCGG
MaeII 533 ACGT A^CGT
761 ACGT
MboII 560 TCTTC GAAGA(8/7)
751 TCTTC
NlaIII 264 CATG CATG^
591 CATG
SacII 605 CCGCGG CCGC^GG
737 CCGCGG
SfcI 220 CTACAG C^T[GA][CT]AG
643 CTGCAG
SmaI 615 CCCGGG CCC^GGG
667 CCCGGG
StyI 473 CCAAGG C^C[AT][AT]GG
590 CCATGG
XmaCI 615 CCCGGG C^CCGGG
667 CCCGGG
XmaI 615 CCCGGG C^CCGGG
667 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI BanI BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV RsaI Sau96I ScrFI TseI