Restriction map for
pF1KB7557
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BbeI BbrPI BbvCI
BbvI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI Fnu4HI FseI HaeII HapII
HgaI Hin1I HpaI HpaII ItaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RsaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TseI
TspRI Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 558 GTCGAC GT^[AC][GT]AC
AclI 144 AACGTT AA^CGTT
AcsI 346 AAATTC [GA]^AATT[CT]
AlwI 441 GATCC GGATC(4/5)
ApaI 483 GGGCCC GGGCC^C
ApoI 346 AAATTC [GA]^AATT[CT]
AspHI 397 GAGCAC G[AT]GC[AT]^C
AviII 408 TGCGCA TGC^GCA
BanI 43 GGCACC G^G[CT][GA]CC
BanII 483 GGGCCC G[GA]GC[CT]^C
BbsI 285 GAAGAC GAAGAC(2/6)
BcgI 140 GCAAAACGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 112 AGATCT A^GATCT
BpuAI 285 GAAGAC GAAGAC(2/6)
BsaBI 325 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 451 GAGGAG GAGGAG(10/8)
BsiHKAI 397 GAGCAC G[AT]GC[AT]^C
BsmAI 540 GTCTC GTCTC(1/5)
BsmFI 234 GGGAC GGGAC(10/14)
BsmI 551 GCATTC GAATGC(1/-1)
BspHI 437 TCATGA T^CATGA
BspMI 386 ACCTGC ACCTGC(4/8)
BstYI 112 AGATCT [GA]^GATC[CT]
CfoI 409 GCGC GCG^C
DsaI 517 CCATGG C^C[GA][CT]GG
EarI 394 GAAGAG CTCTTC(1/4)
EcoNI 444 CCTTGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 408 TGCGCA TGC^GCA
HaeIII 484 GGCC GG^CC
HhaI 409 GCGC GCG^C
HinP1I 409 GCGC G^CGC
HindIII 258 AAGCTT A^AGCTT
HinfI 523 GAATC G^A[ACGT]TC
HphI 493 TCACC GGTGA(8/7)
Ksp632I 394 GAAGAG CTCTTC(1/4)
MaeIII 593 GTGAC ^GT[ACGT]AC
MamI 325 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 112 AGATCT [GA]^GATC[CT]
MseI 293 TTAA T^TAA
MslI 45 CACCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 478 CAGCTG C[AC]G^C[GT]G
NcoI 517 CCATGG C^CATGG
PflMI 130 CCACGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 234 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 144 AACGTT AA^CGTT
PvuII 478 CAGCTG CAG^CTG
RcaI 437 TCATGA T^CATGA
SalI 558 GTCGAC G^TCGAC
SapI 394 GAAGAGC GCTCTTC(1/4)
SfcI 209 CTACAG C^T[GA][CT]AG
TfiI 523 GAATC G^A[AT]TC
Tru9I 293 TTAA T^TAA
Tsp45I 593 GTGAC ^GT[GC]AC
Van91I 130 CCACGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 366 TCTAGA T^CTAGA
XhoII 112 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 85 GGACC G^G[AT]CC
235 GGACC
BfaI 179 CTAG C^TAG
367 CTAG
BmyI 397 GAGCAC G[AGT]GC[ACT]^C
483 GGGCCC
Bsp1286I 397 GAGCAC G[AGT]GC[ACT]^C
483 GGGCCC
BssKI 40 CCAGG ^CC[ACGT]GG
564 CCTGG
BstF5I 63 GGATG GGATG(2/0)
567 GGATG
BstNI 40 CCAGG CC^[AT]GG
564 CCTGG
DraII 234 GGGACCC [GA]G^G[ACGT]CC[CT]
483 GGGCCCC
EcoO109I 234 GGGACCC [GA]G^G[ACGT]CC[CT]
483 GGGCCCC
EcoRII 40 CCAGG ^CC[AT]GG
564 CCTGG
EcoT14I 230 CCAAGG C^C[AT][AT]GG
517 CCATGG
FokI 63 GGATG GGATG(9/13)
567 GGATG
HincII 199 GTCAAC GT[CT]^[GA]AC
558 GTCGAC
HindII 199 GTCAAC GT[CT]^[GA]AC
558 GTCGAC
MaeI 179 CTAG C^TAG
367 CTAG
MaeII 132 ACGT A^CGT
145 ACGT
MvaI 40 CCAGG CC^[AT]GG
564 CCTGG
NlaIII 438 CATG CATG^
518 CATG
ScrFI 40 CCAGG CC^[ACGT]GG
564 CCTGG
SmlI 116 CTCAAG C^T[CT][GA]AG
420 CTTGAG
StyI 230 CCAAGG C^C[AT][AT]GG
517 CCATGG
TaqI 149 TCGA T^CGA
559 TCGA
Tsp509I 347 AATT ^AATT
575 AATT
TthHB8I 149 TCGA T^CGA
559 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BsaJI BsiYI BslI Cfr13I
DdeI DpnII MboI MboII MnlI
MwoI NdeII NlaIV Sau3AI Sau96I
SfaNI