Restriction map for
pF1KB7550
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AvrII BalI BamHI BbeI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstF5I BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FokI FseI HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluNI MroI MscI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 424 GACGTC GACGT^C
AcyI 424 GACGTC G[GA]^CG[CT]C
AlwI 585 GATCC GGATC(4/5)
Aor51HI 554 AGCGCT AGC^GCT
ApaI 223 GGGCCC GGGCC^C
AvaI 220 CCCGGG C^[CT]CG[GA]G
BanI 434 GGCACC G^G[CT][GA]CC
BbrPI 39 CACGTG CAC^GTG
BbsI 253 GAAGAC GAAGAC(2/6)
BfaI 203 CTAG C^TAG
BglI 671 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 86 AGATCT A^GATCT
BpuAI 253 GAAGAC GAAGAC(2/6)
BsaHI 424 GACGTC G[GA]^CG[CT]C
BsmBI 33 CGTCTC CGTCTC(1/5)
BsoBI 220 CCCGGG C^[CT]CG[GA]G
BspMI 355 ACCTGC ACCTGC(4/8)
BsrBI 619 GAGCGG CCGCTC(-3/-3)
BstAPI 443 GCACGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 318 GGTGACC G^GT[ACGT]ACC
BstPI 318 GGTGACC G^GT[ACGT]ACC
BstXI 737 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 665 CTGAG C^T[ACGT]AG
DraII 223 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 554 AGCGCT AGC^GCT
EcoNI 777 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 223 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 318 GGTGACC G^GT[ACGT]ACC
HaeII 554 AGCGCT [GA]GCGC^[CT]
HgaI 32 GCGTC GACGC(5/10)
Hin1I 424 GACGTC G[GA]^CG[CT]C
MaeI 203 CTAG C^TAG
MluI 608 ACGCGT A^CGCGT
NspI 392 GCATGC [GA]CATG^[CT]
PmaCI 39 CACGTG CAC^GTG
PmlI 39 CACGTG CAC^GTG
PstI 781 CTGCAG CTGCA^G
PvuII 241 CAGCTG CAG^CTG
SacI 61 GAGCTC GAGCT^C
SmaI 220 CCCGGG CCC^GGG
SphI 392 GCATGC GCATG^C
XmaCI 220 CCCGGG C^CCGGG
XmaI 220 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 372 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
456 GAAGGGCTTC
AspHI 61 GAGCTC G[AT]GC[AT]^C
700 GAGCAC
AviII 302 TGCGCA TGC^GCA
725 TGCGCA
BanII 61 GAGCTC G[GA]GC[CT]^C
223 GGGCCC
BpmI 54 CTGGAG CTGGAG(16/14)
565 CTGGAG
BsaI 287 GAGACC GGTCTC(1/5)
344 GAGACC
BseRI 73 GAGGAG GAGGAG(10/8)
646 GAGGAG
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
700 GAGCAC
BsmFI 329 GGGAC GGGAC(10/14)
335 GTCCC
BstYI 86 AGATCT [GA]^GATC[CT]
584 AGATCC
DpnII 87 GATC ^GATC
585 GATC
EaeI 152 TGGCCG [CT]^GGCC[GA]
746 TGGCCG
FspI 302 TGCGCA TGC^GCA
725 TGCGCA
HinfI 199 GACTC G^A[ACGT]TC
568 GAGTC
MboI 87 GATC ^GATC
585 GATC
MflI 86 AGATCT [GA]^GATC[CT]
584 AGATCC
MslI 306 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
504 CACTGGCGTG
NcoI 291 CCATGG C^CATGG
588 CCATGG
NdeII 87 GATC ^GATC
585 GATC
PleI 199 GACTC GAGTC(4/5)
568 GAGTC
Sau3AI 87 GATC ^GATC
585 GATC
SmlI 481 CTCAAG C^T[CT][GA]AG
577 CTCAAG
StuI 374 AGGCCT AGG^CCT
542 AGGCCT
TaqI 195 TCGA T^CGA
476 TCGA
TthHB8I 195 TCGA T^CGA
476 TCGA
XhoII 86 AGATCT [GA]^GATC[CT]
584 AGATCC
XmnI 372 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
456 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
BbvI BcnI BmyI BsaAI BsaJI
BsgI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DsaI EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfcI
StyI TseI Tsp45I TspRI