Restriction map for
pF1KB7536
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
AspEI AviII AvrII BalI BamHI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DpnII DraI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tsp509I
Van91I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 702 GACGTC GACGT^C
Acc65I 390 GGTACC G^GTACC
Alw44I 589 GTGCAC G^TGCAC
AlwNI 400 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 589 GTGCAC G^TGCAC
Asp700I 528 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 390 GGTACC G^GTACC
AspI 702 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 523 CTCGAG C^[CT]CG[GA]G
AvaII 659 GGTCC G^G[AT]CC
BbrPI 265 CACGTG CAC^GTG
BbsI 100 GTCTTC GAAGAC(2/6)
BfaI 34 CTAG C^TAG
BpuAI 100 GTCTTC GAAGAC(2/6)
BsaAI 265 CACGTG [CT]AC^GT[GA]
BsaI 541 GAGACC GGTCTC(1/5)
BsaWI 723 ACCGGA [AT]^CCGG[AT]
BsmAI 541 GAGAC GTCTC(1/5)
BsoBI 523 CTCGAG C^[CT]CG[GA]G
BsrFI 69 GCCGGT [GA]^CCGG[CT]
BstAPI 396 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 30 CATCC GGATG(2/0)
Cfr10I 69 GCCGGT [GA]^CCGG[CT]
DrdI 702 GACGTCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 30 CATCC GGATG(9/13)
HphI 721 TCACC GGTGA(8/7)
KpnI 390 GGTACC GGTAC^C
KspI 212 CCGCGG CCGC^GG
MaeI 34 CTAG C^TAG
MaeIII 720 GTCAC ^GT[ACGT]AC
MseI 623 TTAA T^TAA
MslI 414 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 11 GCATGT [GA]CATG^[CT]
PaeR7I 523 CTCGAG C^TCGAG
PflFI 702 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 265 CACGTG CAC^GTG
PmlI 265 CACGTG CAC^GTG
PstI 445 CTGCAG CTGCA^G
SacII 212 CCGCGG CCGC^GG
SfcI 445 CTGCAG C^T[GA][CT]AG
SmlI 523 CTCGAG C^T[CT][GA]AG
Tru9I 623 TTAA T^TAA
Tsp45I 720 GTCAC ^GT[GC]AC
Tth111I 702 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 523 CTCGAG C^TCGAG
XmnI 528 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 391 GTAC GT^AC
549 GTAC
ApaI 285 GGGCCC GGGCC^C
363 GGGCCC
AspHI 330 GTGCTC G[AT]GC[AT]^C
589 GTGCAC
BanII 285 GGGCCC G[GA]GC[CT]^C
363 GGGCCC
BsiEI 43 CGGCCG CG[GA][CT]^CG
209 CGGCCG
BsiHKAI 330 GTGCTC G[AT]GC[AT]^C
589 GTGCAC
BstNI 352 CCAGG CC^[AT]GG
537 CCAGG
DraII 362 AGGGCCC [GA]G^G[ACGT]CC[CT]
363 GGGCCCC
EaeI 43 CGGCCG [CT]^GGCC[GA]
209 CGGCCG
EagI 43 CGGCCG C^GGCCG
209 CGGCCG
EclXI 43 CGGCCG C^GGCCG
209 CGGCCG
Eco52I 43 CGGCCG C^GGCCG
209 CGGCCG
EcoNI 531 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
594 CCTCCGCGAGG
EcoO109I 362 AGGGCCC [GA]G^G[ACGT]CC[CT]
363 GGGCCCC
EcoRII 352 CCAGG ^CC[AT]GG
537 CCAGG
MvaI 352 CCAGG CC^[AT]GG
537 CCAGG
NlaIII 12 CATG CATG^
414 CATG
RsaI 391 GTAC GT^AC
549 GTAC
SfaNI 402 GATGC GCATC(5/9)
20 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbeI BbvI BcnI BmyI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BssKI BstUI Cac8I
CfoI Cfr13I DsaI EarI FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI Ksp632I MaeII MboII
MnlI MspA1I MspI MvnI MwoI
NarI NciI NlaIV PleI PvuII
Sau96I ScrFI SfoI TaqI TseI
TspRI TthHB8I