Restriction map for
pF1KB7523
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# NO CUT ENZYMES ( 201 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvaI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AccII 358 CGCG CG^CG
Alw44I 399 GTGCAC G^TGCAC
ApaLI 399 GTGCAC G^TGCAC
AspHI 399 GTGCAC G[AT]GC[AT]^C
BamHI 152 GGATCC G^GATCC
BsiHKAI 399 GTGCAC G[AT]GC[AT]^C
BsmI 314 GCATTC GAATGC(1/-1)
BsrI 63 ACTGG ACTGG(1/-1)
BstF5I 66 GGATG GGATG(2/0)
BstUI 358 CGCG CG^CG
BstXI 34 CCAGATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 152 GGATCC [GA]^GATC[CT]
DraIII 404 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 170 CCCGC CCCGC(4/6)
FokI 66 GGATG GGATG(9/13)
HinfI 130 GAATC G^A[ACGT]TC
MflI 152 GGATCC [GA]^GATC[CT]
MseI 236 TTAA T^TAA
MvnI 358 CGCG CG^CG
NlaIII 109 CATG CATG^
NlaIV 152 GGATCC GG[ACGT]^[ACGT]CC
SspI 227 AATATT AAT^ATT
TfiI 130 GAATC G^A[AT]TC
Tru9I 236 TTAA T^TAA
Tsp509I 71 AATT ^AATT
XcmI 365 CCAAAAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 152 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 93 AGCT AG^CT
374 AGCT
AlwI 153 GATCC GGATC(4/5)
152 GGATC
BbvI 302 GCTGC GCAGC(8/12)
422 GCTGC
BmyI 361 GTGCCC G[AGT]GC[ACT]^C
399 GTGCAC
BsmFI 326 GGGAC GGGAC(10/14)
340 GGGAC
Bsp1286I 361 GTGCCC G[AGT]GC[ACT]^C
399 GTGCAC
Cfr13I 140 GGCCC G^G[ACGT]CC
384 GGGCC
DpnII 76 GATC ^GATC
153 GATC
HaeIII 140 GGCC GG^CC
385 GGCC
HapII 21 CCGG C^CGG
428 CCGG
HpaII 21 CCGG C^CGG
428 CCGG
MboI 76 GATC ^GATC
153 GATC
MboII 146 TCTTC GAAGA(8/7)
179 TCTTC
MspI 21 CCGG C^CGG
428 CCGG
NdeII 76 GATC ^GATC
153 GATC
Sau3AI 76 GATC ^GATC
153 GATC
Sau96I 140 GGCCC G^G[ACGT]CC
384 GGGCC
SfaNI 350 GCATC GCATC(5/9)
389 GCATC
TaqI 182 TCGA T^CGA
395 TCGA
TseI 302 GCTGC G^C[AT]GC
422 GCTGC
TspRI 62 CACTG CA[GC]TG[ACGT][ACGT]^
406 CACTG
TthHB8I 182 TCGA T^CGA
395 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AciI BsiYI BslI Cac8I Fnu4HI
ItaI MnlI MwoI