Restriction map for
pF1KB7513
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI DdeI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HindIII HinfI
HpaI HphI KasI KpnI Ksp632I
MaeI MamI MfeI MflI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 316 GACGCC G[GA]^CG[CT]C
AfaI 72 GTAC GT^AC
AluI 151 AGCT AG^CT
Alw44I 339 GTGCAC G^TGCAC
ApaLI 339 GTGCAC G^TGCAC
AspHI 339 GTGCAC G[AT]GC[AT]^C
BanII 4 GAGCCC G[GA]GC[CT]^C
BbsI 82 GAAGAC GAAGAC(2/6)
BbvI 152 GCTGC GCAGC(8/12)
BcnI 25 CCGGG CC^[GC]GG
BpuAI 82 GAAGAC GAAGAC(2/6)
BsaAI 334 TACGTG [CT]AC^GT[GA]
BsaHI 316 GACGCC G[GA]^CG[CT]C
BsiHKAI 339 GTGCAC G[AT]GC[AT]^C
BsmI 98 GCATTC GAATGC(1/-1)
BsrI 291 CCAGT ACTGG(1/-1)
CpoI 238 CGGACCG CG^G[AT]CCG
DpnII 171 GATC ^GATC
DraII 221 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 235 CCGCGG C^C[GA][CT]GG
EcoO109I 221 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 77 CCAAGG C^C[AT][AT]GG
FauI 306 GCGGG CCCGC(4/6)
HgaI 316 GACGC GACGC(5/10)
Hin1I 316 GACGCC G[GA]^CG[CT]C
HincII 163 GTCAAC GT[CT]^[GA]AC
HindII 163 GTCAAC GT[CT]^[GA]AC
KspI 235 CCGCGG CCGC^GG
MboI 171 GATC ^GATC
MluI 110 ACGCGT A^CGCGT
NciI 25 CCGGG CC^[GC]GG
NdeII 171 GATC ^GATC
NspI 65 ACATGC [GA]CATG^[CT]
PpuMI 221 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 150 CAGCTG CAG^CTG
RsaI 72 GTAC GT^AC
RsrII 238 CGGACCG CG^G[AT]CCG
SacII 235 CCGCGG CCGC^GG
Sau3AI 171 GATC ^GATC
SfaNI 155 GCATC GCATC(5/9)
StyI 77 CCAAGG C^C[AT][AT]GG
TseI 152 GCTGC G^C[AT]GC
Tsp45I 294 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BmyI 4 GAGCCC G[AGT]GC[ACT]^C
339 GTGCAC
BsmFI 13 GGGAC GGGAC(10/14)
216 GTCCC
Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C
339 GTGCAC
BstNI 51 CCAGG CC^[AT]GG
219 CCAGG
Cac8I 112 GCGTGC GC[ACGT]^[ACGT]GC
306 GCGGGC
CfoI 304 GCGC GCG^C
324 GCGC
DraIII 178 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
296 CACACAGTG
EaeI 267 CGGCCA [CT]^GGCC[GA]
357 CGGCCA
EcoRII 51 CCAGG ^CC[AT]GG
219 CCAGG
HapII 25 CCGG C^CGG
117 CCGG
HhaI 304 GCGC GCG^C
324 GCGC
HinP1I 304 GCGC G^CGC
324 GCGC
HpaII 25 CCGG C^CGG
117 CCGG
MaeIII 59 GTAAC ^GT[ACGT]AC
294 GTCAC
MboII 82 GAAGA GAAGA(8/7)
287 TCTTC
MspI 25 CCGG C^CGG
117 CCGG
MvaI 51 CCAGG CC^[AT]GG
219 CCAGG
NlaIII 66 CATG CATG^
321 CATG
NlaIV 3 GGAGCC GG[ACGT]^[ACGT]CC
222 GGACCC
TspRI 300 CAGTG CA[GC]TG[ACGT][ACGT]^
352 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AccII AciI AflIII AvaII BsaJI
BsiYI BslI BssKI BstUI Cfr13I
Fnu4HI HaeIII ItaI MaeII MnlI
MspA1I MvnI MwoI Sau96I ScrFI