Restriction map for
pF1KB7427
##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspHI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstF5I BstNI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoRV
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII ItaI KasI
Ksp632I KspI MaeII MamI MboI
MfeI MflI MluI MroI MspA1I
MspI MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TseI
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 271 GGTACC G^GTACC
AciI 37 GCGG CCGC(-3/-1)
AcsI 226 GAATTC [GA]^AATT[CT]
AfaI 272 GTAC GT^AC
ApoI 226 GAATTC [GA]^AATT[CT]
AseI 339 ATTAAT AT^TAAT
AsnI 339 ATTAAT AT^TAAT
Asp718I 271 GGTACC G^GTACC
AspI 48 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 356 TGGCCA TGG^CCA
BanI 271 GGTACC G^G[CT][GA]CC
BbsI 163 GAAGAC GAAGAC(2/6)
BfaI 94 CTAG C^TAG
BpuAI 163 GAAGAC GAAGAC(2/6)
BsaI 278 GAGACC GGTCTC(1/5)
BsaJI 359 CCAAGG C^C[ACGT][ACGT]GG
BstBI 104 TTCGAA TT^CGAA
BstEII 26 GGTCACC G^GT[ACGT]ACC
BstPI 26 GGTCACC G^GT[ACGT]ACC
Cac8I 153 GCTTGC GC[ACGT]^[ACGT]GC
DdeI 332 CTCAG C^T[ACGT]AG
EaeI 356 TGGCCA [CT]^GGCC[GA]
EcoO65I 26 GGTCACC G^GT[ACGT]ACC
EcoRI 226 GAATTC G^AATTC
EcoT14I 359 CCAAGG C^C[AT][AT]GG
HaeIII 357 GGCC GG^CC
HinfI 364 GATTC G^A[ACGT]TC
HphI 28 TCACC GGTGA(8/7)
KpnI 271 GGTACC GGTAC^C
MaeI 94 CTAG C^TAG
MaeIII 27 GTCAC ^GT[ACGT]AC
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MslI 311 CACTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 104 TTCGAA TT^CGAA
PflFI 48 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 339 ATTAAT AT^TAAT
RsaI 272 GTAC GT^AC
SfcI 21 CTACAG C^T[GA][CT]AG
SfuI 104 TTCGAA TT^CGAA
StyI 359 CCAAGG C^C[AT][AT]GG
TfiI 364 GATTC G^A[AT]TC
Tsp45I 27 GTCAC ^GT[GC]AC
TspRI 5 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 48 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 93 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AluI 87 AGCT AG^CT
237 AGCT
BsmAI 278 GAGAC GTCTC(1/5)
350 GTCTC
BsrI 405 ACTGG ACTGG(1/-1)
4 CCAGT
MboII 133 GAAGA GAAGA(8/7)
163 GAAGA
MwoI 79 GCCAACCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
199 GCTTCCAAAGC
NlaIII 50 CATG CATG^
354 CATG
SfaNI 97 GATGC GCATC(5/9)
241 GATGC
SspI 108 AATATT AAT^ATT
295 AATATT
TaqI 105 TCGA T^CGA
375 TCGA
TthHB8I 105 TCGA T^CGA
375 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
MnlI MseI NlaIV Tru9I Tsp509I