Restriction map for
pF1KB7426
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MfeI
MflI MluI MroI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AlwI 587 GGATC GGATC(4/5)
AlwNI 6 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 661 GGGCCC GGGCC^C
AspHI 481 GTGCTC G[AT]GC[AT]^C
BalI 413 TGGCCA TGG^CCA
BbsI 1224 GAAGAC GAAGAC(2/6)
BbvCI 786 CCTCAGC CCTCAGC(-5/-2)
BlpI 393 GCTCAGC GC^T[ACGT]AGC
BpmI 1181 CTGGAG CTGGAG(16/14)
Bpu1102I 393 GCTCAGC GC^T[ACGT]AGC
BpuAI 1224 GAAGAC GAAGAC(2/6)
BsaAI 346 TACGTG [CT]AC^GT[GA]
BsaBI 180 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 667 GAGACC GGTCTC(1/5)
BsiHKAI 481 GTGCTC G[AT]GC[AT]^C
BsmI 700 GAATGC GAATGC(1/-1)
BsrI 1180 ACTGG ACTGG(1/-1)
CelII 393 GCTCAGC GC^T[ACGT]AGC
DraI 1099 TTTAAA TTT^AAA
MamI 180 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
NotI 834 GCGGCCGC GC^GGCCGC
NspI 203 ACATGC [GA]CATG^[CT]
PpuMI 542 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 721 CAGCTG CAG^CTG
ScaI 1001 AGTACT AGT^ACT
SmaI 564 CCCGGG CCC^GGG
Tsp509I 924 AATT ^AATT
XcmI 218 CCAACTTGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 564 CCCGGG C^CCGGG
XmaI 564 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AluI 722 AGCT AG^CT
818 AGCT
Aor51HI 404 AGCGCT AGC^GCT
1213 AGCGCT
BbeI 455 GGCGCC GGCGC^C
636 GGCGCC
BseRI 594 CTCCTC GAGGAG(10/8)
1184 GAGGAG
BsgI 360 CTGCAC GTGCAG(16/14)
379 CTGCAC
BssHII 467 GCGCGC G^CGCGC
728 GCGCGC
DpnII 186 GATC ^GATC
588 GATC
DraII 542 AGGACCC [GA]G^G[ACGT]CC[CT]
660 GGGGCCC
Eco47III 404 AGCGCT AGC^GCT
1213 AGCGCT
EcoO109I 542 AGGACCC [GA]G^G[ACGT]CC[CT]
660 GGGGCCC
HinfI 953 GAATC G^A[ACGT]TC
976 GATTC
KasI 455 GGCGCC G^GCGCC
636 GGCGCC
KspI 671 CCGCGG CCGC^GG
680 CCGCGG
MboI 186 GATC ^GATC
588 GATC
MseI 1007 TTAA T^TAA
1100 TTAA
MslI 237 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
474 CATCGCTGTG
NaeI 704 GCCGGC GCC^GGC
830 GCCGGC
NarI 455 GGCGCC GG^CGCC
636 GGCGCC
NdeII 186 GATC ^GATC
588 GATC
NgoMIV 704 GCCGGC G^CCGGC
830 GCCGGC
SacII 671 CCGCGG CCGC^GG
680 CCGCGG
Sau3AI 186 GATC ^GATC
588 GATC
SfoI 455 GGCGCC GGC^GCC
636 GGCGCC
TfiI 953 GAATC G^A[AT]TC
976 GATTC
Tru9I 1007 TTAA T^TAA
1100 TTAA
TspRI 1155 CACTG CA[GC]TG[ACGT][ACGT]^
1179 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AfaI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstEII
BstF5I BstNI BstPI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EagI EclXI Eco52I EcoO65I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TthHB8I