Restriction map for
pF1KB7397
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AflII AgeI AhdI AluI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbrPI BcgI
BciVI BcnI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BsaBI
BseAI BseRI BsiEI BsiHKAI BsmI
BsoBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FseI FspI HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 192 GTATAC GT^[AC][GT]AC
AccII 42 CGCG CG^CG
AcsI 117 AAATTT [GA]^AATT[CT]
AcyI 102 GGCGCC G[GA]^CG[CT]C
AflIII 389 ACACGT A^C[GA][CT]GT
ApoI 117 AAATTT [GA]^AATT[CT]
Asp700I 225 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 158 GGACC G^G[AT]CC
BanI 102 GGCGCC G^G[CT][GA]CC
BbeI 102 GGCGCC GGCGC^C
BbsI 523 GAAGAC GAAGAC(2/6)
BbvCI 362 CCTCAGC CCTCAGC(-5/-2)
BclI 500 TGATCA T^GATCA
BglII 74 AGATCT A^GATCT
BpmI 433 CTGGAG CTGGAG(16/14)
BpuAI 523 GAAGAC GAAGAC(2/6)
BsaAI 390 CACGTA [CT]AC^GT[GA]
BsaHI 102 GGCGCC G[GA]^CG[CT]C
BsaI 54 GGTCTC GGTCTC(1/5)
BsaWI 13 ACCGGA [AT]^CCGG[AT]
BsgI 9 CTGCAC GTGCAG(16/14)
BsiWI 392 CGTACG C^GTACG
BsmBI 396 CGTCTC CGTCTC(1/5)
BsmFI 157 GGGAC GGGAC(10/14)
Bsp1407I 303 TGTACA T^GTACA
BsrGI 303 TGTACA T^GTACA
BssHII 41 GCGCGC G^CGCGC
Bst1107I 192 GTATAC GTA^TAC
BstEII 216 GGTCACC G^GT[ACGT]ACC
BstF5I 441 CATCC GGATG(2/0)
BstPI 216 GGTCACC G^GT[ACGT]ACC
BstUI 42 CGCG CG^CG
BstYI 74 AGATCT [GA]^GATC[CT]
BstZ17I 192 GTATAC GTA^TAC
EarI 347 GAAGAG CTCTTC(1/4)
EcoO65I 216 GGTCACC G^GT[ACGT]ACC
FbaI 500 TGATCA T^GATCA
FokI 441 CATCC GGATG(9/13)
HaeII 102 GGCGCC [GA]GCGC^[CT]
HaeIII 332 GGCC GG^CC
HapII 14 CCGG C^CGG
HgaI 17 GACGC GACGC(5/10)
Hin1I 102 GGCGCC G[GA]^CG[CT]C
HincII 418 GTTGAC GT[CT]^[GA]AC
HindII 418 GTTGAC GT[CT]^[GA]AC
HinfI 263 GACTC G^A[ACGT]TC
HpaII 14 CCGG C^CGG
HphI 218 TCACC GGTGA(8/7)
KasI 102 GGCGCC G^GCGCC
Ksp632I 347 GAAGAG CTCTTC(1/4)
MflI 74 AGATCT [GA]^GATC[CT]
MspI 14 CCGG C^CGG
MvnI 42 CGCG CG^CG
MwoI 11 GCACCGGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 102 GGCGCC GG^CGCC
PflMI 426 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 263 GACTC GAGTC(4/5)
PstI 46 CTGCAG CTGCA^G
SfcI 46 CTGCAG C^T[GA][CT]AG
SfoI 102 GGCGCC GGC^GCC
SspBI 303 TGTACA T^GTACA
TaqI 177 TCGA T^CGA
TthHB8I 177 TCGA T^CGA
Van91I 426 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 74 AGATCT [GA]^GATC[CT]
XmnI 225 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 427 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
571 CAGATGCTG
BsiYI 106 CCTATTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
426 CCAGTACCTGG
BslI 106 CCTATTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
426 CCAGTACCTGG
BsmAI 55 GTCTC GTCTC(1/5)
397 GTCTC
BsrI 96 CCAGT ACTGG(1/-1)
426 CCAGT
Cac8I 23 GCTCGC GC[ACGT]^[ACGT]GC
41 GCGCGC
Cfr13I 158 GGACC G^G[ACGT]CC
331 GGGCC
DdeI 57 CTCAG C^T[ACGT]AG
363 CTCAG
DsaI 369 CCATGG C^C[GA][CT]GG
512 CCATGG
MaeII 391 ACGT A^CGT
395 ACGT
MaeIII 217 GTCAC ^GT[ACGT]AC
403 GTCAC
MslI 183 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
530 CATATTCATG
NcoI 369 CCATGG C^CATGG
512 CCATGG
NlaIV 102 GGCGCC GG[ACGT]^[ACGT]CC
157 GGGACC
Sau96I 158 GGACC G^G[ACGT]CC
331 GGGCC
SfaNI 573 GATGC GCATC(5/9)
413 GCATC
Tsp45I 217 GTCAC ^GT[GC]AC
403 GTCAC
Tsp509I 118 AATT ^AATT
582 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI BbvI BsaJI BssKI BstNI
CfoI DpnII EcoRII EcoT14I Fnu4HI
HhaI HinP1I ItaI MboI MboII
MnlI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI StyI TseI TspRI