##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BpmI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        KasI        KspI        MaeI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        293  GGTACC               G^GTACC
Asp718I       293  GGTACC               G^GTACC
AspHI         627  GAGCAC               G[AT]GC[AT]^C
AvaI          541  CTCGAG               C^[CT]CG[GA]G
AvaII         331  GGTCC                G^G[AT]CC
BanI          293  GGTACC               G^G[CT][GA]CC
BclI          393  TGATCA               T^GATCA
BlpI          624  GCTGAGC              GC^T[ACGT]AGC
BmyI          627  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      624  GCTGAGC              GC^T[ACGT]AGC
BsaJI         166  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       627  GAGCAC               G[AT]GC[AT]^C
BsoBI         541  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      627  GAGCAC               G[AGT]GC[ACT]^C
BsrDI          17  GCAATG               GCAATG(2/0)
CelII         624  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        331  GGTCC                G^G[ACGT]CC
DpnII         394  GATC                 ^GATC
DraI          466  TTTAAA               TTT^AAA
EcoT14I       166  CCTTGG               C^C[AT][AT]GG
FbaI          393  TGATCA               T^GATCA
HaeIII        154  GGCC                 GG^CC
KpnI          293  GGTACC               GGTAC^C
MboI          394  GATC                 ^GATC
MspA1I        622  CAGCTG               C[AC]G^C[GT]G
NdeII         394  GATC                 ^GATC
PaeR7I        541  CTCGAG               C^TCGAG
PvuII         622  CAGCTG               CAG^CTG
Sau3AI        394  GATC                 ^GATC
Sau96I        331  GGTCC                G^G[ACGT]CC
StyI          166  CCTTGG               C^C[AT][AT]GG
TfiI          516  GATTC                G^A[AT]TC
Tsp509I         8  AATT                 ^AATT
XhoI          541  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BseRI          75  CTCCTC               GAGGAG(10/8)
              526  GAGGAG              
BsrI          561  ACTGG                ACTGG(1/-1)
              346  CCAGT               
BstF5I         24  CATCC                GGATG(2/0)
              143  GGATG               
EarI          415  GAAGAG               CTCTTC(1/4)
              547  GAAGAG              
FokI           24  CATCC                GGATG(9/13)
              143  GGATG               
Ksp632I       415  GAAGAG               CTCTTC(1/4)
              547  GAAGAG              
NlaIV         293  GGTACC               GG[ACGT]^[ACGT]CC
              330  GGGTCC              
PleI          618  GACTC                GAGTC(4/5)
              443  GAGTC               
SfaNI          23  GCATC                GCATC(5/9)
              504  GCATC               
SmlI           65  CTCAAG               C^T[CT][GA]AG
              541  CTCGAG              
TaqI          500  TCGA                 T^CGA
              542  TCGA                
Tsp45I        158  GTCAC                ^GT[GC]AC
              203  GTGAC               
TspRI         199  CACTG                CA[GC]TG[ACGT][ACGT]^
              611  CACTG               
TthHB8I       500  TCGA                 T^CGA
              542  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AfaI        AluI        BbvI        DdeI        Fnu4HI      
HinfI       ItaI        MaeIII      MboII       MnlI        
MseI        MwoI        PstI        RsaI        SfcI        
Tru9I       TseI