##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MspI MunI NaeI
NarI NciI NcoI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 17 CGCG CG^CG
AcsI 604 AAATTC [GA]^AATT[CT]
AfaI 228 GTAC GT^AC
Aor51HI 98 AGCGCT AGC^GCT
ApoI 604 AAATTC [GA]^AATT[CT]
Asp700I 342 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 437 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 342 GAAGAC GAAGAC(2/6)
BlpI 490 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 490 GCTGAGC GC^T[ACGT]AGC
BpuAI 342 GAAGAC GAAGAC(2/6)
BsaI 358 GAGACC GGTCTC(1/5)
BseRI 263 CTCCTC GAGGAG(10/8)
BsgI 255 CTGCAC GTGCAG(16/14)
BsiEI 436 CGACCG CG[GA][CT]^CG
BsmAI 358 GAGAC GTCTC(1/5)
Bsp1407I 227 TGTACA T^GTACA
BsrGI 227 TGTACA T^GTACA
BstF5I 111 CATCC GGATG(2/0)
BstUI 17 CGCG CG^CG
CelII 490 GCTGAGC GC^T[ACGT]AGC
CfoI 99 GCGC GCG^C
Eco47III 98 AGCGCT AGC^GCT
FauI 125 GCGGG CCCGC(4/6)
FokI 111 CATCC GGATG(9/13)
HaeII 98 AGCGCT [GA]GCGC^[CT]
HhaI 99 GCGC GCG^C
HinP1I 99 GCGC G^CGC
MaeII 518 ACGT A^CGT
MvnI 17 CGCG CG^CG
NdeI 337 CATATG CA^TATG
NspI 129 GCATGC [GA]CATG^[CT]
PflFI 437 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 228 GTAC GT^AC
SacI 54 GAGCTC GAGCT^C
SfaNI 110 GCATC GCATC(5/9)
SphI 129 GCATGC GCATG^C
SspBI 227 TGTACA T^GTACA
Tth111I 437 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 342 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AspHI 54 GAGCTC G[AT]GC[AT]^C
136 GAGCAC
AvaII 8 GGACC G^G[AT]CC
187 GGACC
BsiHKAI 54 GAGCTC G[AT]GC[AT]^C
136 GAGCAC
EcoT14I 298 CCAAGG C^C[AT][AT]GG
315 CCAAGG
HinfI 483 GAGTC G^A[ACGT]TC
503 GAGTC
PflMI 166 CCATGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
298 CCAAGGACTGG
PleI 483 GAGTC GAGTC(4/5)
503 GAGTC
StyI 298 CCAAGG C^C[AT][AT]GG
315 CCAAGG
Tsp509I 427 AATT ^AATT
605 AATT
TspRI 351 CACTG CA[GC]TG[ACGT][ACGT]^
593 CAGTG
Van91I 166 CCATGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
298 CCAAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HphI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI