Restriction map for
pF1KB7392
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII BalI BamHI BbrPI
BcgI BciVI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
Sau96I SbfI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tsp45I Tth111I
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 135 GACGTC GACGT^C
AccII 321 CGCG CG^CG
AccIII 660 TCCGGA T^CCGGA
AspHI 286 GAGCTC G[AT]GC[AT]^C
AvrII 804 CCTAGG C^CTAGG
BanI 858 GGCGCC G^G[CT][GA]CC
BbeI 858 GGCGCC GGCGC^C
BbvCI 868 GCTGAGG CCTCAGC(-5/-2)
BclI 95 TGATCA T^GATCA
BlnI 804 CCTAGG C^CTAGG
BpmI 970 CTCCAG CTGGAG(16/14)
BsaWI 660 TCCGGA [AT]^CCGG[AT]
BseAI 660 TCCGGA T^CCGGA
BsgI 812 CTGCAC GTGCAG(16/14)
BsiHKAI 286 GAGCTC G[AT]GC[AT]^C
BsmAI 930 GTCTC GTCTC(1/5)
BsmFI 89 GGGAC GGGAC(10/14)
BspEI 660 TCCGGA T^CCGGA
BsrBI 313 GAGCGG CCGCTC(-3/-3)
BsrFI 947 GCCGGT [GA]^CCGG[CT]
BssSI 280 CACGAG CACGAG(-5/-1)
BstUI 321 CGCG CG^CG
BstYI 808 GGATCT [GA]^GATC[CT]
Cfr10I 947 GCCGGT [GA]^CCGG[CT]
DsaI 595 CCACGG C^C[GA][CT]GG
EaeI 598 CGGCCA [CT]^GGCC[GA]
EcoRI 38 GAATTC G^AATTC
EcoRV 65 GATATC GAT^ATC
EcoT14I 804 CCTAGG C^C[AT][AT]GG
FauI 87 GCGGG CCCGC(4/6)
FbaI 95 TGATCA T^GATCA
HgaI 322 GCGTC GACGC(5/10)
HphI 403 TCACC GGTGA(8/7)
KasI 858 GGCGCC G^GCGCC
MflI 808 GGATCT [GA]^GATC[CT]
MroI 660 TCCGGA T^CCGGA
MseI 914 TTAA T^TAA
MslI 417 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 321 CGCG CG^CG
NarI 858 GGCGCC GG^CGCC
SacI 286 GAGCTC GAGCT^C
SapI 942 GAAGAGC GCTCTTC(1/4)
ScaI 613 AGTACT AGT^ACT
SfaNI 250 GATGC GCATC(5/9)
SfoI 858 GGCGCC GGC^GCC
SmaI 308 CCCGGG CCC^GGG
SmlI 298 CTCAAG C^T[CT][GA]AG
SspI 463 AATATT AAT^ATT
StuI 753 AGGCCT AGG^CCT
StyI 804 CCTAGG C^C[AT][AT]GG
TaqI 150 TCGA T^CGA
Tru9I 914 TTAA T^TAA
TspRI 336 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 150 TCGA T^CGA
XhoII 808 GGATCT [GA]^GATC[CT]
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 38 GAATTC [GA]^AATT[CT]
544 AAATTT
AcyI 135 GACGTC G[GA]^CG[CT]C
858 GGCGCC
AfaI 276 GTAC GT^AC
614 GTAC
ApoI 38 GAATTC [GA]^AATT[CT]
544 AAATTT
BbsI 235 GAAGAC GAAGAC(2/6)
874 GAAGAC
BpuAI 235 GAAGAC GAAGAC(2/6)
874 GAAGAC
BsaHI 135 GACGTC G[GA]^CG[CT]C
858 GGCGCC
BsrI 335 CCAGT ACTGG(1/-1)
604 CCAGT
BstF5I 120 GGATG GGATG(2/0)
249 GGATG
Cac8I 340 GCCAGC GC[ACGT]^[ACGT]GC
632 GCCAGC
FokI 120 GGATG GGATG(9/13)
249 GGATG
HaeII 858 GGCGCC [GA]GCGC^[CT]
865 GGCGCT
HaeIII 599 GGCC GG^CC
754 GGCC
Hin1I 135 GACGTC G[GA]^CG[CT]C
858 GGCGCC
MaeIII 524 GTAAC ^GT[ACGT]AC
680 GTAAC
MspA1I 85 CAGCGG C[AC]G^C[GT]G
634 CAGCGG
NlaIV 180 GGAACC GG[ACGT]^[ACGT]CC
858 GGCGCC
PflMI 16 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
240 CCAGGACATGG
RsaI 276 GTAC GT^AC
614 GTAC
TfiI 12 GAATC G^A[AT]TC
448 GAATC
Van91I 16 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
240 CCAGGACATGG
XbaI 325 TCTAGA T^CTAGA
375 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AvaI BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiYI BslI BsoBI Bsp1286I
BssKI BstNI CfoI DdeI DpnII
EarI EcoRII Fnu4HI HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII PleI Sau3AI ScrFI TseI
Tsp509I