Restriction map for
pF1KB7391
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 280 ACATGT A^C[GA][CT]GT
AlwNI 349 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 205 CCTAAGG CC^T[ACGT]AGG
AvrII 229 CCTAGG C^CTAGG
BanI 687 GGTGCC G^G[CT][GA]CC
BcgI 467 GCACCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 684 CCCGG CC^[GC]GG
BfaI 230 CTAG C^TAG
BlnI 229 CCTAGG C^CTAGG
BmyI 688 GTGCCC G[AGT]GC[ACT]^C
BpmI 569 CTGGAG CTGGAG(16/14)
BsaI 47 GGTCTC GGTCTC(1/5)
BseRI 400 GAGGAG GAGGAG(10/8)
BsmI 94 GAATGC GAATGC(1/-1)
Bsp1286I 688 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 280 ACATGT A^CATGT
BspMI 469 ACCTGC ACCTGC(4/8)
BsrFI 454 ACCGGC [GA]^CCGG[CT]
BstAPI 31 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 205 CCTAAGG CC^T[ACGT]AGG
Cfr10I 454 ACCGGC [GA]^CCGG[CT]
Cfr13I 525 GGGCC G^G[ACGT]CC
DdeI 206 CTAAG C^T[ACGT]AG
DrdI 654 GACTGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 205 CCTAAGG CC^T[ACGT]AGG
EcoT14I 229 CCTAGG C^C[AT][AT]GG
EcoT22I 419 ATGCAT ATGCA^T
MaeI 230 CTAG C^TAG
MboII 21 TCTTC GAAGA(8/7)
MseI 7 TTAA T^TAA
MspA1I 380 CCGCTG C[AC]G^C[GT]G
NciI 684 CCCGG CC^[GC]GG
NlaIV 687 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 419 ATGCAT ATGCA^T
Sau96I 525 GGGCC G^G[ACGT]CC
StyI 229 CCTAGG C^C[AT][AT]GG
Tru9I 7 TTAA T^TAA
TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 681 GATCC GGATC(4/5)
188 GGATC
BsgI 168 CTGCAC GTGCAG(16/14)
303 CTGCAC
BsrI 650 ACTGG ACTGG(1/-1)
616 CCAGT
BstYI 188 GGATCT [GA]^GATC[CT]
680 AGATCC
Cac8I 305 GCACGC GC[ACGT]^[ACGT]GC
354 GCTGGC
DpnII 189 GATC ^GATC
681 GATC
EaeI 462 TGGCCG [CT]^GGCC[GA]
597 TGGCCG
FauI 379 CCCGC CCCGC(4/6)
495 GCGGG
HinfI 55 GAATC G^A[ACGT]TC
667 GATTC
MboI 189 GATC ^GATC
681 GATC
MflI 188 GGATCT [GA]^GATC[CT]
680 AGATCC
NdeII 189 GATC ^GATC
681 GATC
NspI 78 ACATGC [GA]CATG^[CT]
280 ACATGT
PstI 68 CTGCAG CTGCA^G
447 CTGCAG
Sau3AI 189 GATC ^GATC
681 GATC
SfcI 68 CTGCAG C^T[GA][CT]AG
447 CTGCAG
TfiI 55 GAATC G^A[AT]TC
667 GATTC
XhoII 188 GGATCT [GA]^GATC[CT]
680 AGATCC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsmAI
BsmFI BssKI BstF5I BstNI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MnlI MspI MvaI
MwoI NlaIII ScrFI SfaNI TseI
Tsp509I