Restriction map for
pF1KB7387
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcnI
BfrI BglI BlpI Bpu1102I BpuAI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MunI
MvaI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 204 CGCG CG^CG
AciI 1017 CCGC CCGC(-3/-1)
Alw44I 1190 GTGCAC G^TGCAC
AlwNI 369 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1190 GTGCAC G^TGCAC
AspHI 1190 GTGCAC G[AT]GC[AT]^C
AvaI 1223 CTCGGG C^[CT]CG[GA]G
AvaII 99 GGACC G^G[AT]CC
AvrII 387 CCTAGG C^CTAGG
BcgI 1254 GCAAACTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 714 TGATCA T^GATCA
BglII 40 AGATCT A^GATCT
BlnI 387 CCTAGG C^CTAGG
BmyI 1190 GTGCAC G[AGT]GC[ACT]^C
BsaAI 646 TACGTA [CT]AC^GT[GA]
BsaWI 1054 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1190 GTGCAC G[AT]GC[AT]^C
BsiYI 1218 CCATACTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1218 CCATACTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 134 GAGACG CGTCTC(1/5)
BsmFI 98 GGGAC GGGAC(10/14)
BsmI 936 GCATTC GAATGC(1/-1)
BsoBI 1223 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1190 GTGCAC G[AGT]GC[ACT]^C
BsrDI 908 GCAATG GCAATG(2/0)
BstUI 204 CGCG CG^CG
BstYI 40 AGATCT [GA]^GATC[CT]
CfoI 205 GCGC GCG^C
Cfr13I 99 GGACC G^G[ACGT]CC
DsaI 74 CCGTGG C^C[GA][CT]GG
EaeI 71 TGGCCG [CT]^GGCC[GA]
EcoT14I 387 CCTAGG C^C[AT][AT]GG
FbaI 714 TGATCA T^GATCA
HapII 1055 CCGG C^CGG
HhaI 205 GCGC GCG^C
HinP1I 205 GCGC G^CGC
HincII 297 GTCAAC GT[CT]^[GA]AC
HindII 297 GTCAAC GT[CT]^[GA]AC
HindIII 1007 AAGCTT A^AGCTT
HpaII 1055 CCGG C^CGG
MaeIII 460 GTAAC ^GT[ACGT]AC
MflI 40 AGATCT [GA]^GATC[CT]
MslI 262 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 103 CAGCTG C[AC]G^C[GT]G
MspI 1055 CCGG C^CGG
MvnI 204 CGCG CG^CG
NlaIV 98 GGGACC GG[ACGT]^[ACGT]CC
PstI 728 CTGCAG CTGCA^G
PvuII 103 CAGCTG CAG^CTG
Sau96I 99 GGACC G^G[ACGT]CC
SmlI 499 CTTGAG C^T[CT][GA]AG
SnaBI 646 TACGTA TAC^GTA
SspI 313 AATATT AAT^ATT
StuI 755 AGGCCT AGG^CCT
StyI 387 CCTAGG C^C[AT][AT]GG
TaqI 132 TCGA T^CGA
TthHB8I 132 TCGA T^CGA
XhoII 40 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 108 GTAC GT^AC
378 GTAC
BbvI 549 GCAGC GCAGC(8/12)
1283 GCAGC
BciVI 255 GGATAC GTATCC(6/5)
854 GGATAC
BpmI 145 CTGGAG CTGGAG(16/14)
971 CTGGAG
BsaJI 74 CCGTGG C^C[ACGT][ACGT]GG
387 CCTAGG
BseRI 4 GAGGAG GAGGAG(10/8)
91 GAGGAG
Cac8I 248 GCCTGC GC[ACGT]^[ACGT]GC
782 GCCAGC
HaeIII 72 GGCC GG^CC
756 GGCC
MwoI 183 GCTTTGTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
207 GCAGTTGGAGC
NlaIII 337 CATG CATG^
1123 CATG
RsaI 108 GTAC GT^AC
378 GTAC
SfcI 153 CTACAG C^T[GA][CT]AG
728 CTGCAG
TseI 549 GCAGC G^C[AT]GC
1283 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BfaI
BsaBI BsmAI BsrI BstF5I DdeI
DpnII EcoRI Fnu4HI FokI HinfI
HphI ItaI MaeI MaeII MamI
MboI MboII MnlI MseI NdeII
PleI Sau3AI SfaNI TfiI Tru9I
Tsp509I TspRI XmnI