Restriction map for
pF1KB7386
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI KspI MaeIII
MamI MfeI MluI MroI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI StuI
Tsp45I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 76 CGCG CG^CG
Alw44I 1215 GTGCAC G^TGCAC
ApaLI 1215 GTGCAC G^TGCAC
Asp700I 505 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1215 GTGCAC G[AT]GC[AT]^C
BalI 803 TGGCCA TGG^CCA
BamHI 625 GGATCC G^GATCC
BanI 1185 GGCACC G^G[CT][GA]CC
BmyI 1215 GTGCAC G[AGT]GC[ACT]^C
BpmI 425 CTCCAG CTGGAG(16/14)
BsiHKAI 1215 GTGCAC G[AT]GC[AT]^C
Bsp1286I 1215 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 993 TGTACA T^GTACA
BsrGI 993 TGTACA T^GTACA
BssKI 806 CCAGG ^CC[ACGT]GG
BstNI 806 CCAGG CC^[AT]GG
BstUI 76 CGCG CG^CG
Cac8I 929 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 75 GCGC GCG^C
DraIII 887 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 677 CCATGG C^C[GA][CT]GG
EaeI 803 TGGCCA [CT]^GGCC[GA]
EcoRII 806 CCAGG ^CC[AT]GG
EcoT14I 677 CCATGG C^C[AT][AT]GG
FauI 54 GCGGG CCCGC(4/6)
HaeIII 804 GGCC GG^CC
HhaI 75 GCGC GCG^C
HinP1I 75 GCGC G^CGC
HindIII 212 AAGCTT A^AGCTT
MaeII 526 ACGT A^CGT
MluNI 803 TGGCCA TGG^CCA
MscI 803 TGGCCA TGG^CCA
MslI 66 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 683 CAGCTG C[AC]G^C[GT]G
MvaI 806 CCAGG CC^[AT]GG
MvnI 76 CGCG CG^CG
NcoI 677 CCATGG C^CATGG
PflMI 615 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 88 CTGCAG CTGCA^G
PvuII 683 CAGCTG CAG^CTG
SbfI 87 CCTGCAGG CCTGCA^GG
ScrFI 806 CCAGG CC^[ACGT]GG
Sse8387I 87 CCTGCAGG CCTGCA^GG
SspBI 993 TGTACA T^GTACA
SspI 351 AATATT AAT^ATT
StyI 677 CCATGG C^C[AT][AT]GG
SwaI 495 ATTTAAAT ATTT^AAAT
Van91I 615 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1189 CCAAAAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 505 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 60 CCGC CCGC(-3/-1)
54 GCGG
AvaII 57 GGACC G^G[AT]CC
84 GGACC
BbsI 13 GAAGAC GAAGAC(2/6)
921 GTCTTC
BpuAI 13 GAAGAC GAAGAC(2/6)
921 GTCTTC
BsaJI 677 CCATGG C^C[ACGT][ACGT]GG
806 CCAGGG
BseRI 948 CTCCTC GAGGAG(10/8)
951 CTCCTC
BsgI 94 GTGCAG GTGCAG(16/14)
662 GTGCAG
BsmFI 56 GGGAC GGGAC(10/14)
723 GGGAC
BspMI 86 ACCTGC ACCTGC(4/8)
90 GCAGGT
BsrDI 103 GCAATG GCAATG(2/0)
1225 GCAATG
BstF5I 188 CATCC GGATG(2/0)
1123 GGATG
BstYI 48 GGATCT [GA]^GATC[CT]
625 GGATCC
Cfr13I 57 GGACC G^G[ACGT]CC
84 GGACC
EcoRI 133 GAATTC G^AATTC
606 GAATTC
FokI 188 CATCC GGATG(9/13)
1123 GGATG
MflI 48 GGATCT [GA]^GATC[CT]
625 GGATCC
NlaIII 409 CATG CATG^
678 CATG
PleI 883 GACTC GAGTC(4/5)
125 GAGTC
SapI 175 GAAGAGC GCTCTTC(1/4)
508 GCTCTTC
Sau96I 57 GGACC G^G[ACGT]CC
84 GGACC
SmlI 279 CTTGAG C^T[CT][GA]AG
689 CTCAAG
TfiI 1030 GAATC G^A[AT]TC
1036 GATTC
XhoII 48 GGATCT [GA]^GATC[CT]
625 GGATCC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BfaI BsiYI BslI
BsmI BsrI DdeI DpnII DraI
EarI Fnu4HI HinfI HphI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MwoI NdeII NlaIV RsaI
Sau3AI SfaNI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I