Restriction map for
pF1KB7385
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 225 GTCTAC GT^[AC][GT]AC
AcyI 289 GACGCC G[GA]^CG[CT]C
AluI 34 AGCT AG^CT
AocI 700 CCTGAGG CC^T[ACGT]AGG
AvaII 475 GGACC G^G[AT]CC
BcnI 456 CCGGG CC^[GC]GG
BfaI 707 CTAG C^TAG
BlpI 606 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 606 GCTGAGC GC^T[ACGT]AGC
BsaAI 373 TACGTG [CT]AC^GT[GA]
BsaHI 289 GACGCC G[GA]^CG[CT]C
BsgI 169 CTGCAC GTGCAG(16/14)
BspLU11I 571 ACATGT A^CATGT
BsrDI 771 CATTGC GCAATG(2/0)
BstYI 902 AGATCC [GA]^GATC[CT]
Bsu36I 700 CCTGAGG CC^T[ACGT]AGG
CelII 606 GCTGAGC GC^T[ACGT]AGC
CfoI 731 GCGC GCG^C
DrdI 580 GACAGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 356 CCATGG C^C[GA][CT]GG
Eco81I 700 CCTGAGG CC^T[ACGT]AGG
HapII 456 CCGG C^CGG
HgaI 289 GACGC GACGC(5/10)
HhaI 731 GCGC GCG^C
Hin1I 289 GACGCC G[GA]^CG[CT]C
HinP1I 731 GCGC G^CGC
HpaII 456 CCGG C^CGG
MaeI 707 CTAG C^TAG
MflI 902 AGATCC [GA]^GATC[CT]
MseI 713 TTAA T^TAA
MslI 739 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 456 CCGG C^CGG
NciI 456 CCGGG CC^[GC]GG
NcoI 356 CCATGG C^CATGG
NlaIV 100 GGCTCC GG[ACGT]^[ACGT]CC
SfcI 557 CTGTAG C^T[GA][CT]AG
Tru9I 713 TTAA T^TAA
XhoII 902 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 495 CCGC CCGC(-3/-1)
501 GCGG
AflIII 75 ACACGT A^C[GA][CT]GT
571 ACATGT
AspHI 409 GTGCTC G[AT]GC[AT]^C
547 GAGCAC
BalI 377 TGGCCA TGG^CCA
836 TGGCCA
BmyI 409 GTGCTC G[AGT]GC[ACT]^C
547 GAGCAC
BpmI 818 CTCCAG CTGGAG(16/14)
64 CTGGAG
BsiHKAI 409 GTGCTC G[AT]GC[AT]^C
547 GAGCAC
BsmFI 724 GGGAC GGGAC(10/14)
69 GTCCC
Bsp1286I 409 GTGCTC G[AGT]GC[ACT]^C
547 GAGCAC
BsrI 49 ACTGG ACTGG(1/-1)
820 CCAGT
EaeI 377 TGGCCA [CT]^GGCC[GA]
836 TGGCCA
EcoT14I 356 CCATGG C^C[AT][AT]GG
928 CCAAGG
HphI 738 TCACC GGTGA(8/7)
792 TCACC
MaeIII 133 GTGAC ^GT[ACGT]AC
275 GTGAC
MluNI 377 TGGCCA TGG^CCA
836 TGGCCA
MscI 377 TGGCCA TGG^CCA
836 TGGCCA
MwoI 556 GCTGTAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
646 GCTATTGCTGC
SexAI 110 ACCTGGT A^CC[AT]GGT
215 ACCTGGT
StyI 356 CCATGG C^C[AT][AT]GG
928 CCAAGG
TfiI 254 GAATC G^A[AT]TC
789 GATTC
Tsp45I 133 GTGAC ^GT[GC]AC
275 GTGAC
XcmI 104 CCATGTACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
137 CCATCCTGGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AlwI BbvI BsaJI BseRI
BsiYI BslI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeII MboI MboII MnlI
MvaI NdeII NlaIII NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI TspRI