Restriction map for
pF1KB7377
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstUI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 778 GTATAC GT^[AC][GT]AC
AcyI 439 GGCGTC G[GA]^CG[CT]C
Alw44I 591 GTGCAC G^TGCAC
AlwNI 1048 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 48 GGGCCC GGGCC^C
ApaLI 591 GTGCAC G^TGCAC
BamHI 193 GGATCC G^GATCC
BciVI 480 GTATCC GTATCC(6/5)
BglII 266 AGATCT A^GATCT
BsaAI 604 TACGTG [CT]AC^GT[GA]
BsaHI 439 GGCGTC G[GA]^CG[CT]C
BsmFI 1278 GGGAC GGGAC(10/14)
BsmI 766 GAATGC GAATGC(1/-1)
BsrDI 581 GCAATG GCAATG(2/0)
BssSI 676 CTCGTG CACGAG(-5/-1)
Bst1107I 778 GTATAC GTA^TAC
BstAPI 503 GCATGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 645 CCACTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 778 GTATAC GTA^TAC
DrdI 447 GACTGGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 586 GAAGAG CTCTTC(1/4)
HgaI 440 GCGTC GACGC(5/10)
Hin1I 439 GGCGTC G[GA]^CG[CT]C
Ksp632I 586 GAAGAG CTCTTC(1/4)
PflMI 66 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 878 ACCAGGT A^CC[AT]GGT
SmaI 870 CCCGGG CCC^GGG
SmlI 1035 CTCAAG C^T[CT][GA]AG
StuI 746 AGGCCT AGG^CCT
Tsp509I 1000 AATT ^AATT
Van91I 66 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 870 CCCGGG C^CCGGG
XmaI 870 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AspHI 97 GTGCTC G[AT]GC[AT]^C
591 GTGCAC
AvaI 870 CCCGGG C^[CT]CG[GA]G
1275 CTCGGG
BalI 392 TGGCCA TGG^CCA
785 TGGCCA
BbsI 706 GTCTTC GAAGAC(2/6)
1232 GTCTTC
BfaI 140 CTAG C^TAG
229 CTAG
BpuAI 706 GTCTTC GAAGAC(2/6)
1232 GTCTTC
BsgI 1063 CTGCAC GTGCAG(16/14)
165 GTGCAG
BsiHKAI 97 GTGCTC G[AT]GC[AT]^C
591 GTGCAC
BsmAI 681 GTCTC GTCTC(1/5)
1185 GTCTC
BsoBI 870 CCCGGG C^[CT]CG[GA]G
1275 CTCGGG
BstYI 193 GGATCC [GA]^GATC[CT]
266 AGATCT
DraIII 159 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
514 CACTGGGTG
EcoT14I 71 CCATGG C^C[AT][AT]GG
399 CCATGG
HapII 525 CCGG C^CGG
871 CCGG
HpaII 525 CCGG C^CGG
871 CCGG
MaeI 140 CTAG C^TAG
229 CTAG
MaeII 545 ACGT A^CGT
605 ACGT
MaeIII 941 GTGAC ^GT[ACGT]AC
1030 GTGAC
MflI 193 GGATCC [GA]^GATC[CT]
266 AGATCT
MluNI 392 TGGCCA TGG^CCA
785 TGGCCA
MscI 392 TGGCCA TGG^CCA
785 TGGCCA
MspI 525 CCGG C^CGG
871 CCGG
NcoI 71 CCATGG C^CATGG
399 CCATGG
NspI 354 GCATGT [GA]CATG^[CT]
387 GCATGT
PpuMI 461 GGGTCCT [GA]G^G[AT]CC[CT]
1278 GGGACCC
PstI 246 CTGCAG CTGCA^G
697 CTGCAG
PvuII 168 CAGCTG CAG^CTG
1120 CAGCTG
SfcI 246 CTGCAG C^T[GA][CT]AG
697 CTGCAG
StyI 71 CCATGG C^C[AT][AT]GG
399 CCATGG
TaqI 254 TCGA T^CGA
965 TCGA
Tsp45I 941 GTGAC ^GT[GC]AC
1030 GTGAC
TthHB8I 254 TCGA T^CGA
965 TCGA
XhoII 193 GGATCC [GA]^GATC[CT]
266 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HphI
ItaI MboI MboII MnlI MslI
MspA1I MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI TspRI