Restriction map for
pF1KB7376
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HincII HindII HpaI HphI
KasI KpnI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Alw44I 960 GTGCAC G^TGCAC
AlwNI 262 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 213 CCTCAGG CC^T[ACGT]AGG
Aor51HI 198 AGCGCT AGC^GCT
ApaLI 960 GTGCAC G^TGCAC
AspHI 960 GTGCAC G[AT]GC[AT]^C
AvaI 724 CCCGAG C^[CT]CG[GA]G
AvaII 474 GGACC G^G[AT]CC
BbsI 219 GAAGAC GAAGAC(2/6)
BclI 947 TGATCA T^GATCA
BcnI 155 CCCGG CC^[GC]GG
BfaI 797 CTAG C^TAG
BglI 187 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 960 GTGCAC G[AGT]GC[ACT]^C
BpuAI 219 GAAGAC GAAGAC(2/6)
BsiHKAI 960 GTGCAC G[AT]GC[AT]^C
BsmFI 168 GTCCC GGGAC(10/14)
BsoBI 724 CCCGAG C^[CT]CG[GA]G
Bsp1286I 960 GTGCAC G[AGT]GC[ACT]^C
BspMI 261 GCAGGT ACCTGC(4/8)
BsrFI 648 GCCGGC [GA]^CCGG[CT]
Bsu36I 213 CCTCAGG CC^T[ACGT]AGG
Cfr10I 648 GCCGGC [GA]^CCGG[CT]
DraI 880 TTTAAA TTT^AAA
EaeI 536 CGGCCA [CT]^GGCC[GA]
Eco47III 198 AGCGCT AGC^GCT
Eco81I 213 CCTCAGG CC^T[ACGT]AGG
EcoT14I 442 CCTTGG C^C[AT][AT]GG
FbaI 947 TGATCA T^GATCA
HindIII 762 AAGCTT A^AGCTT
HinfI 282 GAGTC G^A[ACGT]TC
MaeI 797 CTAG C^TAG
MseI 881 TTAA T^TAA
NaeI 648 GCCGGC GCC^GGC
NciI 155 CCCGG CC^[GC]GG
NgoMIV 648 GCCGGC G^CCGGC
PleI 282 GAGTC GAGTC(4/5)
PshAI 161 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 130 CTGCAG CTGCA^G
SapI 201 GCTCTTC GCTCTTC(1/4)
SfcI 130 CTGCAG C^T[GA][CT]AG
SphI 357 GCATGC GCATG^C
StyI 442 CCTTGG C^C[AT][AT]GG
Tru9I 881 TTAA T^TAA
Tsp509I 316 AATT ^AATT
XcmI 701 CCAGCTGCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 534 CGCG CG^CG
613 CGCG
AfaI 228 GTAC GT^AC
791 GTAC
BpmI 801 CTCCAG CTGGAG(16/14)
298 CTGGAG
BsaI 967 GAGACC GGTCTC(1/5)
714 GGTCTC
BsmBI 528 CGTCTC CGTCTC(1/5)
159 GAGACG
BstUI 534 CGCG CG^CG
613 CGCG
BstYI 23 AGATCC [GA]^GATC[CT]
731 AGATCC
DsaI 533 CCGCGG C^C[GA][CT]GG
612 CCGCGG
EarI 202 CTCTTC CTCTTC(1/4)
940 GAAGAG
HaeII 198 AGCGCT [GA]GCGC^[CT]
516 GGCGCT
Ksp632I 202 CTCTTC CTCTTC(1/4)
940 GAAGAG
KspI 533 CCGCGG CCGC^GG
612 CCGCGG
MflI 23 AGATCC [GA]^GATC[CT]
731 AGATCC
MvnI 534 CGCG CG^CG
613 CGCG
NspI 357 GCATGC [GA]CATG^[CT]
398 GCATGT
RsaI 228 GTAC GT^AC
791 GTAC
SacII 533 CCGCGG CCGC^GG
612 CCGCGG
SmlI 139 CTTGAG C^T[CT][GA]AG
905 CTCAAG
XhoII 23 AGATCC [GA]^GATC[CT]
731 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoNI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI StuI
TseI TspRI