Restriction map for
pF1KB7375
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 518 AAATTC [GA]^AATT[CT]
AhdI 590 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 467 GGATC GGATC(4/5)
ApoI 518 AAATTC [GA]^AATT[CT]
AspEI 590 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 684 GGGCTC G[GA]GC[CT]^C
BbsI 424 GTCTTC GAAGAC(2/6)
BpuAI 424 GTCTTC GAAGAC(2/6)
Bsp1407I 773 TGTACA T^GTACA
BsrGI 773 TGTACA T^GTACA
Cac8I 477 GCTTGC GC[ACGT]^[ACGT]GC
DrdI 447 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 131 CCATGG C^C[GA][CT]GG
Eam1105I 590 GACTACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 386 CTCTTC CTCTTC(1/4)
HincII 191 GTCAAC GT[CT]^[GA]AC
HindII 191 GTCAAC GT[CT]^[GA]AC
Ksp632I 386 CTCTTC CTCTTC(1/4)
MaeIII 380 GTCAC ^GT[ACGT]AC
MseI 928 TTAA T^TAA
MspA1I 482 CAGCTG C[AC]G^C[GT]G
MwoI 346 GCTGTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 131 CCATGG C^CATGG
PleI 746 GACTC GAGTC(4/5)
PstI 504 CTGCAG CTGCA^G
PvuII 482 CAGCTG CAG^CTG
SfaNI 98 GCATC GCATC(5/9)
SspBI 773 TGTACA T^GTACA
SspI 124 AATATT AAT^ATT
StuI 707 AGGCCT AGG^CCT
TaqI 666 TCGA T^CGA
Tru9I 928 TTAA T^TAA
Tsp45I 380 GTCAC ^GT[GC]AC
Tsp509I 519 AATT ^AATT
TspRI 107 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 666 TCGA T^CGA
XcmI 678 CCACATGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
876 CCGC
BmyI 684 GGGCTC G[AGT]GC[ACT]^C
896 GGGCAC
BsaI 780 GAGACC GGTCTC(1/5)
455 GGTCTC
BsgI 222 CTGCAC GTGCAG(16/14)
564 CTGCAC
BsiYI 363 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
732 CCTGTCTGTGG
BslI 363 CCGCTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
732 CCTGTCTGTGG
BsmAI 780 GAGAC GTCTC(1/5)
456 GTCTC
Bsp1286I 684 GGGCTC G[AGT]GC[ACT]^C
896 GGGCAC
BspHI 404 TCATGA T^CATGA
428 TCATGA
BspMI 718 ACCTGC ACCTGC(4/8)
283 GCAGGT
BsrI 108 ACTGG ACTGG(1/-1)
628 CCAGT
DdeI 187 CTCAG C^T[ACGT]AG
296 CTCAG
DpnII 243 GATC ^GATC
468 GATC
EcoT14I 131 CCATGG C^C[AT][AT]GG
449 CCTTGG
HaeIII 708 GGCC GG^CC
825 GGCC
HphI 381 TCACC GGTGA(8/7)
842 TCACC
MboI 243 GATC ^GATC
468 GATC
NdeII 243 GATC ^GATC
468 GATC
RcaI 404 TCATGA T^CATGA
428 TCATGA
Sau3AI 243 GATC ^GATC
468 GATC
SfcI 504 CTGCAG C^T[GA][CT]AG
901 CTACAG
StyI 131 CCATGG C^C[AT][AT]GG
449 CCTTGG
TfiI 47 GAATC G^A[AT]TC
408 GAATC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BseRI BsmFI BssKI BstF5I BstNI
Cfr13I EcoRII Fnu4HI FokI HinfI
ItaI MboII MnlI MslI MvaI
NlaIII RsaI Sau96I ScrFI TseI