Restriction map for
pF1KB7371
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflII 85 CTTAAG C^TTAAG
AluI 653 AGCT AG^CT
AlwNI 922 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 606 GGGCCC GGGCC^C
AspHI 22 GTGCTC G[AT]GC[AT]^C
AvaI 25 CTCGGG C^[CT]CG[GA]G
BamHI 241 GGATCC G^GATCC
BanI 816 GGTGCC G^G[CT][GA]CC
BcnI 556 CCCGG CC^[GC]GG
BfaI 754 CTAG C^TAG
BfrI 85 CTTAAG C^TTAAG
BglII 479 AGATCT A^GATCT
BseRI 513 CTCCTC GAGGAG(10/8)
BsiHKAI 22 GTGCTC G[AT]GC[AT]^C
BsoBI 25 CTCGGG C^[CT]CG[GA]G
BspMI 727 ACCTGC ACCTGC(4/8)
BssSI 301 CACGAG CACGAG(-5/-1)
BstAPI 921 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 63 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 930 GCGC GCG^C
DrdI 409 GACCCCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 253 CCATGG C^C[AT][AT]GG
FauI 899 CCCGC CCCGC(4/6)
HhaI 930 GCGC GCG^C
HinP1I 930 GCGC G^CGC
MaeI 754 CTAG C^TAG
MslI 543 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 921 GCAGATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 556 CCCGG CC^[GC]GG
NcoI 253 CCATGG C^CATGG
PleI 77 GACTC GAGTC(4/5)
SmlI 85 CTTAAG C^T[CT][GA]AG
StuI 149 AGGCCT AGG^CCT
StyI 253 CCATGG C^C[AT][AT]GG
TfiI 225 GATTC G^A[AT]TC
Tsp45I 167 GTGAC ^GT[GC]AC
XcmI 300 CCACGAGTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 69 GTAC GT^AC
510 GTAC
BanII 379 GGGCTC G[GA]GC[CT]^C
606 GGGCCC
BbsI 117 GTCTTC GAAGAC(2/6)
271 GTCTTC
BpuAI 117 GTCTTC GAAGAC(2/6)
271 GTCTTC
BsgI 462 CTGCAC GTGCAG(16/14)
732 CTGCAC
BsmFI 599 GGGAC GGGAC(10/14)
438 GTCCC
BsrDI 836 CATTGC GCAATG(2/0)
938 CATTGC
BsrI 262 ACTGG ACTGG(1/-1)
888 CCAGT
DdeI 163 CTGAG C^T[ACGT]AG
854 CTAAG
DsaI 253 CCATGG C^C[GA][CT]GG
276 CCACGG
HapII 557 CCGG C^CGG
841 CCGG
HinfI 77 GACTC G^A[ACGT]TC
225 GATTC
HpaII 557 CCGG C^CGG
841 CCGG
MaeII 158 ACGT A^CGT
269 ACGT
MaeIII 167 GTGAC ^GT[ACGT]AC
266 GTTAC
MseI 86 TTAA T^TAA
476 TTAA
MspI 557 CCGG C^CGG
841 CCGG
NlaIII 174 CATG CATG^
254 CATG
RsaI 69 GTAC GT^AC
510 GTAC
TaqI 419 TCGA T^CGA
881 TCGA
Tru9I 86 TTAA T^TAA
476 TTAA
Tsp509I 40 AATT ^AATT
474 AATT
TspRI 330 CAGTG CA[GC]TG[ACGT][ACGT]^
735 CACTG
TthHB8I 419 TCGA T^CGA
881 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AlwI AvaII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cfr13I
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HphI ItaI
Ksp632I MboI MboII MflI MnlI
MvaI NdeII NlaIV PpuMI Sau3AI
Sau96I ScrFI SfaNI TseI XhoII