Restriction map for
pF1KB7370
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I TthHB8I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AciI 486 GCGG CCGC(-3/-1)
AcsI 209 AAATTT [GA]^AATT[CT]
AfaI 59 GTAC GT^AC
AlwI 526 GGATC GGATC(4/5)
ApoI 209 AAATTT [GA]^AATT[CT]
AspI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 161 CCCGAG C^[CT]CG[GA]G
AvaII 12 GGTCC G^G[AT]CC
BanI 389 GGTGCC G^G[CT][GA]CC
BbvI 271 GCAGC GCAGC(8/12)
BglI 320 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 194 GGGCAC G[AGT]GC[ACT]^C
BpmI 492 CTGGAG CTGGAG(16/14)
BsgI 286 GTGCAG GTGCAG(16/14)
BsmAI 253 GAGAC GTCTC(1/5)
BsmBI 253 GAGACG CGTCTC(1/5)
BsmFI 261 GGGAC GGGAC(10/14)
BsoBI 161 CCCGAG C^[CT]CG[GA]G
Bsp1286I 194 GGGCAC G[AGT]GC[ACT]^C
BsrBI 484 GAGCGG CCGCTC(-3/-3)
BstF5I 78 GGATG GGATG(2/0)
BstYI 526 GGATCT [GA]^GATC[CT]
Cac8I 202 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 12 GGTCC G^G[ACGT]CC
DpnII 527 GATC ^GATC
DraII 11 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 47 CCATGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoO109I 11 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 47 CCATGG C^C[AT][AT]GG
Fnu4HI 271 GCAGC GC^[ACGT]GC
FokI 78 GGATG GGATG(9/13)
HaeIII 3 GGCC GG^CC
HindIII 337 AAGCTT A^AGCTT
HphI 52 GGTGA GGTGA(8/7)
ItaI 271 GCAGC GC^[ACGT]GC
MaeII 132 ACGT A^CGT
MaeIII 242 GTTAC ^GT[ACGT]AC
MboI 527 GATC ^GATC
MflI 526 GGATCT [GA]^GATC[CT]
MslI 197 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 47 CCATGG C^CATGG
NdeII 527 GATC ^GATC
NlaIV 389 GGTGCC GG[ACGT]^[ACGT]CC
PflFI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 42 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 11 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 59 GTAC GT^AC
SapI 274 GCTCTTC GCTCTTC(1/4)
Sau3AI 527 GATC ^GATC
Sau96I 12 GGTCC G^G[ACGT]CC
SfaNI 215 GCATC GCATC(5/9)
SfcI 143 CTACAG C^T[GA][CT]AG
SmlI 315 CTCAAG C^T[CT][GA]AG
SphI 202 GCATGC GCATG^C
StyI 47 CCATGG C^C[AT][AT]GG
TseI 271 GCAGC G^C[AT]GC
Tth111I 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 42 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 348 TCTAGA T^CTAGA
XhoII 526 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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BbsI 235 GAAGAC GAAGAC(2/6)
352 GAAGAC
BfaI 349 CTAG C^TAG
539 CTAG
BpuAI 235 GAAGAC GAAGAC(2/6)
352 GAAGAC
BsaJI 47 CCATGG C^C[ACGT][ACGT]GG
546 CCCAGG
BsiYI 42 CCATTCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
547 CCAGGTTTAGG
BslI 42 CCATTCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
547 CCAGGTTTAGG
BsrI 75 ACTGG ACTGG(1/-1)
571 ACTGG
DdeI 267 CTCAG C^T[ACGT]AG
466 CTTAG
HgaI 255 GACGC GACGC(5/10)
355 GACGC
MaeI 349 CTAG C^TAG
539 CTAG
MnlI 387 GAGG CCTC(7/6)
495 GAGG
NspI 202 GCATGC [GA]CATG^[CT]
310 ACATGC
PleI 175 GACTC GAGTC(4/5)
263 GACTC
TfiI 332 GAATC G^A[AT]TC
542 GAATC
TspRI 284 CAGTG CA[GC]TG[ACGT][ACGT]^
454 CAGTG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BssKI BstNI EarI EcoRII
HinfI Ksp632I MboII MvaI MwoI
NlaIII ScrFI Tsp509I