Restriction map for
pF1KB7369
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII ItaI KasI KpnI
KspI MaeII MboI MflI MluI
MroI MspA1I MspI MvnI MwoI
NaeI NarI NciI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI TaqI TseI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AseI 657 ATTAAT AT^TAAT
AsnI 657 ATTAAT AT^TAAT
Asp700I 31 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BlpI 722 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 722 GCTCAGC GC^T[ACGT]AGC
BsaBI 639 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 687 CTCCTC GAGGAG(10/8)
BsmAI 796 GAGAC GTCTC(1/5)
BsmI 414 GAATGC GAATGC(1/-1)
BspMI 718 ACCTGC ACCTGC(4/8)
BsrDI 581 GCAATG GCAATG(2/0)
BssKI 443 CCTGG ^CC[ACGT]GG
BstNI 443 CCTGG CC^[AT]GG
BstXI 20 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 722 GCTCAGC GC^T[ACGT]AGC
DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 766 CTCTTC CTCTTC(1/4)
EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 443 CCTGG ^CC[AT]GG
EcoRV 392 GATATC GAT^ATC
HincII 460 GTCAAC GT[CT]^[GA]AC
HindII 460 GTCAAC GT[CT]^[GA]AC
HindIII 707 AAGCTT A^AGCTT
Ksp632I 766 CTCTTC CTCTTC(1/4)
MaeIII 72 GTTAC ^GT[ACGT]AC
MamI 639 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 493 CAATTG C^AATTG
MunI 493 CAATTG C^AATTG
MvaI 443 CCTGG CC^[AT]GG
PleI 861 GACTC GAGTC(4/5)
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 735 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 657 ATTAAT AT^TAAT
ScrFI 443 CCTGG CC^[ACGT]GG
SpeI 243 ACTAGT A^CTAGT
XmnI 31 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 31 GAATTT [GA]^AATT[CT]
539 AAATTC
ApoI 31 GAATTT [GA]^AATT[CT]
539 AAATTC
BalI 314 TGGCCA TGG^CCA
602 TGGCCA
BfaI 244 CTAG C^TAG
544 CTAG
BsgI 290 GTGCAG GTGCAG(16/14)
295 GTGCAG
BspHI 404 TCATGA T^CATGA
551 TCATGA
DsaI 317 CCATGG C^C[GA][CT]GG
428 CCATGG
EaeI 314 TGGCCA [CT]^GGCC[GA]
602 TGGCCA
MaeI 244 CTAG C^TAG
544 CTAG
MluNI 314 TGGCCA TGG^CCA
602 TGGCCA
MscI 314 TGGCCA TGG^CCA
602 TGGCCA
NcoI 317 CCATGG C^CATGG
428 CCATGG
PstI 467 CTGCAG CTGCA^G
504 CTGCAG
RcaI 404 TCATGA T^CATGA
551 TCATGA
SmlI 67 CTTGAG C^T[CT][GA]AG
676 CTCAAG
SspI 609 AATATT AAT^ATT
747 AATATT
TfiI 681 GATTC G^A[AT]TC
873 GAATC
TspRI 293 CAGTG CA[GC]TG[ACGT][ACGT]^
629 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI AvaII BsaJI BsiYI
BslI BsmFI BstF5I Cfr13I DdeI
EcoT14I FokI HaeIII HinfI HphI
MboII MnlI MseI MslI NlaIII
NlaIV RsaI Sau96I SfaNI SfcI
StyI Tru9I Tsp509I