Restriction map for
pF1KB7368
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII BamHI BanI BbeI
BbrPI BbvCI BclI BcnI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI FauI FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MflI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AfaI 183 GTAC GT^AC
AlwI 366 GGATC GGATC(4/5)
AocI 482 CCTTAGG CC^T[ACGT]AGG
AspHI 547 GAGCAC G[AT]GC[AT]^C
AvrII 627 CCTAGG C^CTAGG
BanII 487 GGGCTC G[GA]GC[CT]^C
BcgI 331 GCAATTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 628 CTAG C^TAG
BglI 196 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 627 CCTAGG C^CTAGG
BpmI 64 CTGGAG CTGGAG(16/14)
BsgI 73 GTGCAG GTGCAG(16/14)
BsiHKAI 547 GAGCAC G[AT]GC[AT]^C
BsmAI 396 GAGAC GTCTC(1/5)
BsmFI 856 GTCCC GGGAC(10/14)
BsmI 233 GCATTC GAATGC(1/-1)
Bsp1407I 182 TGTACA T^GTACA
BspMI 229 ACCTGC ACCTGC(4/8)
BsrGI 182 TGTACA T^GTACA
Bsu36I 482 CCTTAGG CC^T[ACGT]AGG
Cac8I 567 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 360 GGCCC G^G[ACGT]CC
DpnII 367 GATC ^GATC
Eco81I 482 CCTTAGG CC^T[ACGT]AGG
EcoNI 478 CCTTCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 580 GATATC GAT^ATC
EcoT14I 627 CCTAGG C^C[AT][AT]GG
EcoT22I 729 ATGCAT ATGCA^T
HincII 589 GTCAAC GT[CT]^[GA]AC
HindII 589 GTCAAC GT[CT]^[GA]AC
HphI 447 GGTGA GGTGA(8/7)
MaeI 628 CTAG C^TAG
MboI 367 GATC ^GATC
MfeI 332 CAATTG C^AATTG
MslI 739 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 688 CAGCTG C[AC]G^C[GT]G
MunI 332 CAATTG C^AATTG
MwoI 196 GCCATGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 367 GATC ^GATC
NsiI 729 ATGCAT ATGCA^T
PvuII 688 CAGCTG CAG^CTG
RsaI 183 GTAC GT^AC
Sau3AI 367 GATC ^GATC
Sau96I 360 GGCCC G^G[ACGT]CC
SmlI 393 CTTGAG C^T[CT][GA]AG
SspBI 182 TGTACA T^GTACA
SspI 642 AATATT AAT^ATT
StuI 716 AGGCCT AGG^CCT
StyI 627 CCTAGG C^C[AT][AT]GG
TaqI 516 TCGA T^CGA
TthHB8I 516 TCGA T^CGA
XcmI 557 CCATCGTGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 566 AGCT AG^CT
689 AGCT
BciVI 344 GTATCC GTATCC(6/5)
386 GTATCC
BmyI 487 GGGCTC G[AGT]GC[ACT]^C
547 GAGCAC
BsaJI 362 CCCTGG C^C[ACGT][ACGT]GG
627 CCTAGG
BsiYI 478 CCTTCCTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCAGAAGGAGG
BslI 478 CCTTCCTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCAGAAGGAGG
Bsp1286I 487 GGGCTC G[AGT]GC[ACT]^C
547 GAGCAC
NspI 399 ACATGC [GA]CATG^[CT]
727 ACATGC
PleI 67 GAGTC GAGTC(4/5)
587 GAGTC
SfaNI 289 GATGC GCATC(5/9)
254 GCATC
SfcI 521 CTACAG C^T[GA][CT]AG
754 CTACAG
Tsp45I 448 GTGAC ^GT[GC]AC
908 GTGAC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI ApoI BalI BbsI BbvI
BpuAI BsrDI BssKI BstNI DdeI
EaeI EcoRII Fnu4HI HaeIII HinfI
ItaI MaeIII MboII MluNI MnlI
MscI MseI MvaI NlaIII ScrFI
TfiI Tru9I TseI Tsp509I TspRI