Restriction map for
pF1KB7367
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI HphI KasI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 74 GGTACC G^GTACC
AcyI 43 GGCGTC G[GA]^CG[CT]C
AfaI 75 GTAC GT^AC
AluI 338 AGCT AG^CT
Alw44I 205 GTGCAC G^TGCAC
AlwNI 337 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 205 GTGCAC G^TGCAC
Asp718I 74 GGTACC G^GTACC
BanI 74 GGTACC G^G[CT][GA]CC
BbsI 436 GAAGAC GAAGAC(2/6)
BfaI 349 CTAG C^TAG
BpmI 475 CTGGAG CTGGAG(16/14)
BpuAI 436 GAAGAC GAAGAC(2/6)
BsaAI 91 TACGTG [CT]AC^GT[GA]
BsaHI 43 GGCGTC G[GA]^CG[CT]C
BseRI 310 GAGGAG GAGGAG(10/8)
BsmFI 5 GTCCC GGGAC(10/14)
BsrI 461 ACTGG ACTGG(1/-1)
BstAPI 336 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 240 GGATG GGATG(2/0)
CfoI 33 GCGC GCG^C
EaeI 227 TGGCCG [CT]^GGCC[GA]
EcoNI 303 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 240 GGATG GGATG(9/13)
HaeII 32 GGCGCT [GA]GCGC^[CT]
HhaI 33 GCGC GCG^C
Hin1I 43 GGCGTC G[GA]^CG[CT]C
HinP1I 33 GCGC G^CGC
HincII 106 GTCAAC GT[CT]^[GA]AC
HindII 106 GTCAAC GT[CT]^[GA]AC
HinfI 439 GACTC G^A[ACGT]TC
KpnI 74 GGTACC GGTAC^C
KspI 8 CCGCGG CCGC^GG
MaeI 349 CTAG C^TAG
MaeII 92 ACGT A^CGT
MaeIII 378 GTCAC ^GT[ACGT]AC
NlaIII 292 CATG CATG^
PleI 439 GACTC GAGTC(4/5)
PpuMI 3 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 75 GTAC GT^AC
SacII 8 CCGCGG CCGC^GG
Tsp45I 378 GTCAC ^GT[GC]AC
Tsp509I 446 AATT ^AATT
XcmI 126 CCAGCAGCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 46 GTCTAC GT^[AC][GT]AC
88 GTCTAC
AspHI 196 GTGCTC G[AT]GC[AT]^C
205 GTGCAC
BcnI 71 CCCGG CC^[GC]GG
78 CCGGG
BsaI 392 GAGACC GGTCTC(1/5)
478 GAGACC
BsiHKAI 196 GTGCTC G[AT]GC[AT]^C
205 GTGCAC
BsmAI 392 GAGAC GTCTC(1/5)
478 GAGAC
BspMI 50 ACCTGC ACCTGC(4/8)
470 ACCTGC
Cac8I 173 GCGGGC GC[ACGT]^[ACGT]GC
278 GCCTGC
DpnII 297 GATC ^GATC
399 GATC
DsaI 8 CCGCGG C^C[GA][CT]GG
224 CCGTGG
EarI 244 GAAGAG CTCTTC(1/4)
451 GAAGAG
HapII 72 CCGG C^CGG
78 CCGG
HgaI 44 GCGTC GACGC(5/10)
86 GCGTC
HpaII 72 CCGG C^CGG
78 CCGG
Ksp632I 244 GAAGAG CTCTTC(1/4)
451 GAAGAG
MboI 297 GATC ^GATC
399 GATC
MspA1I 8 CCGCGG C[AC]G^C[GT]G
171 CAGCGG
MspI 72 CCGG C^CGG
78 CCGG
NciI 71 CCCGG CC^[GC]GG
78 CCGGG
NdeII 297 GATC ^GATC
399 GATC
Sau3AI 297 GATC ^GATC
399 GATC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI ApaI AvaII BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI BstUI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII ItaI MboII MnlI
MvaI MvnI MwoI NlaIV Sau96I
ScrFI TseI TspRI