Restriction map for
pF1KB7366
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I EarI EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 80 CGCG CG^CG
AfaI 243 GTAC GT^AC
AocI 488 CCTCAGG CC^T[ACGT]AGG
Aor51HI 419 AGCGCT AGC^GCT
AseI 496 ATTAAT AT^TAAT
AsnI 496 ATTAAT AT^TAAT
AspHI 324 GTGCTC G[AT]GC[AT]^C
AvaI 145 CCCGAG C^[CT]CG[GA]G
BamHI 347 GGATCC G^GATCC
BcnI 512 CCGGG CC^[GC]GG
BseRI 667 GAGGAG GAGGAG(10/8)
BsiHKAI 324 GTGCTC G[AT]GC[AT]^C
BsmBI 713 CGTCTC CGTCTC(1/5)
BsmFI 173 GTCCC GGGAC(10/14)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
Bsp1407I 242 TGTACA T^GTACA
BsrBI 615 GAGCGG CCGCTC(-3/-3)
BsrGI 242 TGTACA T^GTACA
BssSI 716 CTCGTG CACGAG(-5/-1)
BstF5I 627 CATCC GGATG(2/0)
BstUI 80 CGCG CG^CG
BstYI 347 GGATCC [GA]^GATC[CT]
Bsu36I 488 CCTCAGG CC^T[ACGT]AGG
Eco47III 419 AGCGCT AGC^GCT
Eco81I 488 CCTCAGG CC^T[ACGT]AGG
FokI 627 CATCC GGATG(9/13)
HincII 358 GTCAAC GT[CT]^[GA]AC
HindII 358 GTCAAC GT[CT]^[GA]AC
MaeII 392 ACGT A^CGT
MaeIII 46 GTGAC ^GT[ACGT]AC
MflI 347 GGATCC [GA]^GATC[CT]
MslI 627 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 80 CGCG CG^CG
NciI 512 CCGGG CC^[GC]GG
NotI 617 GCGGCCGC GC^GGCCGC
PshAI 307 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 496 ATTAAT AT^TAAT
PstI 522 CTGCAG CTGCA^G
RsaI 243 GTAC GT^AC
SspBI 242 TGTACA T^GTACA
StuI 570 AGGCCT AGG^CCT
TaqI 609 TCGA T^CGA
TfiI 730 GATTC G^A[AT]TC
Tsp45I 46 GTGAC ^GT[GC]AC
Tsp509I 481 AATT ^AATT
TthHB8I 609 TCGA T^CGA
XcmI 662 CCAATGAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 347 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaII 93 GGACC G^G[AT]CC
485 GGACC
BalI 162 TGGCCA TGG^CCA
674 TGGCCA
BpmI 551 CTCCAG CTGGAG(16/14)
576 CTGGAG
BsiEI 509 CGGCCG CG[GA][CT]^CG
618 CGGCCG
BsmAI 314 GTCTC GTCTC(1/5)
714 GTCTC
BstAPI 15 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
24 GCAGCTGCTGC
DdeI 489 CTCAG C^T[ACGT]AG
645 CTCAG
DpnII 259 GATC ^GATC
348 GATC
EagI 509 CGGCCG C^GGCCG
618 CGGCCG
EclXI 509 CGGCCG C^GGCCG
618 CGGCCG
Eco52I 509 CGGCCG C^GGCCG
618 CGGCCG
HaeII 290 AGCGCC [GA]GCGC^[CT]
419 AGCGCT
HphI 45 GGTGA GGTGA(8/7)
328 TCACC
MboI 259 GATC ^GATC
348 GATC
MboII 180 GAAGA GAAGA(8/7)
183 GAAGA
MluNI 162 TGGCCA TGG^CCA
674 TGGCCA
MscI 162 TGGCCA TGG^CCA
674 TGGCCA
MseI 251 TTAA T^TAA
497 TTAA
NdeII 259 GATC ^GATC
348 GATC
PleI 643 GACTC GAGTC(4/5)
535 GAGTC
PvuII 25 CAGCTG CAG^CTG
309 CAGCTG
Sau3AI 259 GATC ^GATC
348 GATC
SfaNI 21 GATGC GCATC(5/9)
626 GCATC
SfcI 522 CTGCAG C^T[GA][CT]AG
603 CTATAG
Tru9I 251 TTAA T^TAA
497 TTAA
TspRI 166 CAGTG CA[GC]TG[ACGT][ACGT]^
262 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
BanII BbvI BmyI BsaJI BsgI
BsiYI BslI BsmI Bsp1286I BspMI
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MnlI
MspA1I MspI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TseI