Restriction map for
pF1KB7360
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NdeII NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tsp45I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 776 CGCG CG^CG
AspI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 160 GGGCTC G[GA]GC[CT]^C
BbrPI 714 CACGTG CAC^GTG
BbvI 287 GCTGC GCAGC(8/12)
BcnI 236 CCCGG CC^[GC]GG
BmyI 160 GGGCTC G[AGT]GC[ACT]^C
BsaAI 714 CACGTG [CT]AC^GT[GA]
BseRI 323 GAGGAG GAGGAG(10/8)
BsmAI 57 GTCTC GTCTC(1/5)
BsmFI 541 GTCCC GGGAC(10/14)
Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C
BspHI 404 TCATGA T^CATGA
BspMI 418 ACCTGC ACCTGC(4/8)
BsrDI 427 GCAATG GCAATG(2/0)
BsrFI 380 GCCGGC [GA]^CCGG[CT]
BsrI 54 CCAGT ACTGG(1/-1)
BstUI 776 CGCG CG^CG
CfoI 777 GCGC GCG^C
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
DraIII 823 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 817 CTCTTC CTCTTC(1/4)
EcoRI 31 GAATTC G^AATTC
EcoT22I 209 ATGCAT ATGCA^T
Fnu4HI 287 GCTGC GC^[ACGT]GC
HhaI 777 GCGC GCG^C
HinP1I 777 GCGC G^CGC
HindIII 884 AAGCTT A^AGCTT
HinfI 846 GAATC G^A[ACGT]TC
ItaI 287 GCTGC GC^[ACGT]GC
Ksp632I 817 CTCTTC CTCTTC(1/4)
MaeII 715 ACGT A^CGT
MvnI 776 CGCG CG^CG
NaeI 380 GCCGGC GCC^GGC
NciI 236 CCCGG CC^[GC]GG
NgoMIV 380 GCCGGC G^CCGGC
NlaIV 231 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 209 ATGCAT ATGCA^T
PflFI 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 714 CACGTG CAC^GTG
PmlI 714 CACGTG CAC^GTG
RcaI 404 TCATGA T^CATGA
SfcI 279 CTACAG C^T[GA][CT]AG
SmlI 59 CTCAAG C^T[CT][GA]AG
SspI 96 AATATT AAT^ATT
TfiI 846 GAATC G^A[AT]TC
TseI 287 GCTGC G^C[AT]GC
Tth111I 51 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 467 CCATTATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 363 CCGC CCGC(-3/-1)
492 CCGC
AcsI 31 GAATTC [GA]^AATT[CT]
123 AAATTT
ApoI 31 GAATTC [GA]^AATT[CT]
123 AAATTT
AvaII 480 GGTCC G^G[AT]CC
754 GGACC
BbsI 598 GTCTTC GAAGAC(2/6)
681 GTCTTC
BfaI 88 CTAG C^TAG
413 CTAG
BpuAI 598 GTCTTC GAAGAC(2/6)
681 GTCTTC
BsgI 388 CTGCAC GTGCAG(16/14)
564 CTGCAC
DdeI 296 CTCAG C^T[ACGT]AG
689 CTGAG
EcoT14I 42 CCTTGG C^C[AT][AT]GG
318 CCTTGG
HapII 237 CCGG C^CGG
381 CCGG
HpaII 237 CCGG C^CGG
381 CCGG
HphI 140 TCACC GGTGA(8/7)
722 TCACC
MaeI 88 CTAG C^TAG
413 CTAG
MseI 94 TTAA T^TAA
905 TTAA
MslI 573 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
620 CACTCCTGTG
MspA1I 517 CAGCTG C[AC]G^C[GT]G
797 CAGCTG
MspI 237 CCGG C^CGG
381 CCGG
MwoI 646 GCCTCCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
790 GCTTTCCCAGC
PvuII 517 CAGCTG CAG^CTG
797 CAGCTG
StyI 42 CCTTGG C^C[AT][AT]GG
318 CCTTGG
Tru9I 94 TTAA T^TAA
905 TTAA
Tsp509I 32 AATT ^AATT
124 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwNI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
EcoRII FokI HaeIII MboII MnlI
MvaI NlaIII Sau96I ScrFI SfaNI
TspRI