Restriction map for
pF1KB7358
##################################
# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AvaI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BmyI
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tsp509I
Van91I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 77 GGTACC G^GTACC
Asp718I 77 GGTACC G^GTACC
AspI 1180 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 77 GGTACC G^G[CT][GA]CC
BbsI 1034 GTCTTC GAAGAC(2/6)
BciVI 443 GTATCC GTATCC(6/5)
BglI 140 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 756 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 756 GCTGAGC GC^T[ACGT]AGC
BpuAI 1034 GTCTTC GAAGAC(2/6)
BseRI 1104 CTCCTC GAGGAG(10/8)
BsgI 272 CTGCAC GTGCAG(16/14)
Bsp1407I 1194 TGTACA T^GTACA
BspHI 540 TCATGA T^CATGA
BspMI 80 ACCTGC ACCTGC(4/8)
BsrDI 762 CATTGC GCAATG(2/0)
BsrGI 1194 TGTACA T^GTACA
BssSI 562 CTCGTG CACGAG(-5/-1)
BstYI 312 GGATCT [GA]^GATC[CT]
CelII 756 GCTGAGC GC^T[ACGT]AGC
EcoT22I 360 ATGCAT ATGCA^T
HphI 57 TCACC GGTGA(8/7)
KpnI 77 GGTACC GGTAC^C
MaeII 1154 ACGT A^CGT
MflI 312 GGATCT [GA]^GATC[CT]
MseI 1253 TTAA T^TAA
NheI 493 GCTAGC G^CTAGC
NsiI 360 ATGCAT ATGCA^T
NspI 358 ACATGC [GA]CATG^[CT]
PflFI 1180 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 833 CTGCAG CTGCA^G
RcaI 540 TCATGA T^CATGA
SfcI 833 CTGCAG C^T[GA][CT]AG
SmlI 872 CTCAAG C^T[CT][GA]AG
SspBI 1194 TGTACA T^GTACA
TaqI 598 TCGA T^CGA
Tru9I 1253 TTAA T^TAA
Tth111I 1180 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 598 TCGA T^CGA
XcmI 479 CCATCTGGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 312 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 216 GATCC GGATC(4/5)
312 GGATC
BalI 395 TGGCCA TGG^CCA
476 TGGCCA
BcnI 117 CCCGG CC^[GC]GG
840 CCCGG
BfaI 316 CTAG C^TAG
494 CTAG
BsaBI 171 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1137 GATTCCCATC
BsmFI 1178 GGGAC GGGAC(10/14)
1186 GTCCC
BsrI 101 ACTGG ACTGG(1/-1)
344 ACTGG
Cfr13I 838 GGCCC G^G[ACGT]CC
843 GGCCC
EaeI 395 TGGCCA [CT]^GGCC[GA]
476 TGGCCA
MaeI 316 CTAG C^TAG
494 CTAG
MamI 171 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1137 GATTCCCATC
MluNI 395 TGGCCA TGG^CCA
476 TGGCCA
MscI 395 TGGCCA TGG^CCA
476 TGGCCA
NciI 117 CCCGG CC^[GC]GG
840 CCCGG
Sau96I 838 GGCCC G^G[ACGT]CC
843 GGCCC
Tsp45I 422 GTGAC ^GT[GC]AC
742 GTCAC
TspRI 467 CAGTG CA[GC]TG[ACGT][ACGT]^
746 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI BbvI BpmI BsaJI
BsiYI BslI BsmAI BssKI BstF5I
BstNI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
ScrFI SfaNI TfiI TseI