Restriction map for
pF1KB7303
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# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AccIII AclI AflII AscI
AseI AsnI Asp700I AvrII BamHI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EcoRV FbaI FseI HindIII HpaI
MaeI MfeI MroI MunI NdeI
NheI NotI NruI NspV PacI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 982 GAATTC [GA]^AATT[CT]
AgeI 2508 ACCGGT A^CCGGT
AhdI 2752 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 737 AGCGCT AGC^GCT
ApoI 982 GAATTC [GA]^AATT[CT]
AspEI 2752 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3532 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 677 TGCGCA TGC^GCA
BbvCI 1439 CCTCAGC CCTCAGC(-5/-2)
BsaBI 1177 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2508 ACCGGT [AT]^CCGG[AT]
BspHI 2234 TCATGA T^CATGA
BspLU11I 2897 ACATGT A^CATGT
BsrDI 2241 CATTGC GCAATG(2/0)
BstYI 3446 AGATCC [GA]^GATC[CT]
Eam1105I 2752 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 737 AGCGCT AGC^GCT
EcoRI 982 GAATTC G^AATTC
EcoT22I 1484 ATGCAT ATGCA^T
FspI 677 TGCGCA TGC^GCA
HincII 1300 GTCAAC GT[CT]^[GA]AC
HindII 1300 GTCAAC GT[CT]^[GA]AC
MamI 1177 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3446 AGATCC [GA]^GATC[CT]
MluI 414 ACGCGT A^CGCGT
MseI 97 TTAA T^TAA
NsiI 1484 ATGCAT ATGCA^T
PflFI 3532 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2508 ACCGGT A^CCGGT
PshAI 208 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2234 TCATGA T^CATGA
SacI 2815 GAGCTC GAGCT^C
SgrAI 2507 CACCGGTG C[GA]^CCGG[CT]G
Tru9I 97 TTAA T^TAA
Tsp509I 983 AATT ^AATT
Tth111I 3532 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 3446 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 58 GACGTC GACGT^C
3696 GACGTC
Acc65I 46 GGTACC G^GTACC
3461 GGTACC
AlwI 3447 GATCC GGATC(4/5)
3657 GATCC
AocI 376 CCTGAGG CC^T[ACGT]AGG
1258 CCTGAGG
Asp718I 46 GGTACC G^GTACC
3461 GGTACC
BsmBI 2392 GAGACG CGTCTC(1/5)
3352 GAGACG
BstEII 2130 GGTGACC G^GT[ACGT]ACC
3702 GGTGACC
BstPI 2130 GGTGACC G^GT[ACGT]ACC
3702 GGTGACC
Bsu36I 376 CCTGAGG CC^T[ACGT]AGG
1258 CCTGAGG
EarI 1078 CTCTTC CTCTTC(1/4)
391 GAAGAG
Eco81I 376 CCTGAGG CC^T[ACGT]AGG
1258 CCTGAGG
EcoO65I 2130 GGTGACC G^GT[ACGT]ACC
3702 GGTGACC
KpnI 46 GGTACC GGTAC^C
3461 GGTACC
Ksp632I 1078 CTCTTC CTCTTC(1/4)
391 GAAGAG
KspI 3079 CCGCGG CCGC^GG
3639 CCGCGG
PaeR7I 542 CTCGAG C^TCGAG
1936 CTCGAG
SacII 3079 CCGCGG CCGC^GG
3639 CCGCGG
SphI 179 GCATGC GCATG^C
1482 GCATGC
StuI 1262 AGGCCT AGG^CCT
2849 AGGCCT
TfiI 2409 GATTC G^A[AT]TC
3681 GAATC
XhoI 542 CTCGAG C^TCGAG
1936 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI