Restriction map for
pF1KB7300
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI Asp700I AspEI AviII AvrII
BamHI BbrPI BciVI BfrI BlnI
BsaAI BsaBI BsiWI BsmBI Bsp1407I
BspDI BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1831 GTCTAC GT^[AC][GT]AC
AflIII 803 ACATGT A^C[GA][CT]GT
AseI 900 ATTAAT AT^TAAT
AsnI 900 ATTAAT AT^TAAT
AspI 2484 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 16 GGCGCC GGCGC^C
BbsI 153 GAAGAC GAAGAC(2/6)
BcgI 926 GCACCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2330 TGATCA T^GATCA
BglII 490 AGATCT A^GATCT
BpuAI 153 GAAGAC GAAGAC(2/6)
BsmI 1005 GAATGC GAATGC(1/-1)
BspHI 1793 TCATGA T^CATGA
BspLU11I 803 ACATGT A^CATGT
BsrFI 2513 GCCGGC [GA]^CCGG[CT]
BstEII 301 GGTAACC G^GT[ACGT]ACC
BstPI 301 GGTAACC G^GT[ACGT]ACC
Cfr10I 2513 GCCGGC [GA]^CCGG[CT]
DraI 618 TTTAAA TTT^AAA
EcoO65I 301 GGTAACC G^GT[ACGT]ACC
FbaI 2330 TGATCA T^GATCA
HaeII 16 GGCGCC [GA]GCGC^[CT]
HincII 985 GTCAAC GT[CT]^[GA]AC
HindII 985 GTCAAC GT[CT]^[GA]AC
KasI 16 GGCGCC G^GCGCC
KspI 64 CCGCGG CCGC^GG
NaeI 2513 GCCGGC GCC^GGC
NarI 16 GGCGCC GG^CGCC
NgoMIV 2513 GCCGGC G^CCGGC
PflFI 2484 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 900 ATTAAT AT^TAAT
RcaI 1793 TCATGA T^CATGA
SacII 64 CCGCGG CCGC^GG
SapI 562 GCTCTTC GCTCTTC(1/4)
SfoI 16 GGCGCC GGC^GCC
Tth111I 2484 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 40 enzymes) #
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Acc65I 1510 GGTACC G^GTACC
2740 GGTACC
AccIII 761 TCCGGA T^CCGGA
2340 TCCGGA
AlwNI 552 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1579 CAGCAGCTG
AocI 1350 CCTGAGG CC^T[ACGT]AGG
1843 CCTGAGG
ApaI 2030 GGGCCC GGGCC^C
2461 GGGCCC
Asp718I 1510 GGTACC G^GTACC
2740 GGTACC
AvaI 513 CCCGGG C^[CT]CG[GA]G
2464 CCCGGG
BfaI 2191 CTAG C^TAG
2447 CTAG
BlpI 196 GCTGAGC GC^T[ACGT]AGC
387 GCTCAGC
Bpu1102I 196 GCTGAGC GC^T[ACGT]AGC
387 GCTCAGC
BsaI 686 GAGACC GGTCTC(1/5)
2468 GGTCTC
BsaWI 761 TCCGGA [AT]^CCGG[AT]
2340 TCCGGA
BseAI 761 TCCGGA T^CCGGA
2340 TCCGGA
BsgI 2414 CTGCAC GTGCAG(16/14)
1929 GTGCAG
BsiEI 61 CGGCCG CG[GA][CT]^CG
2569 CGGCCG
BsoBI 513 CCCGGG C^[CT]CG[GA]G
2464 CCCGGG
BspEI 761 TCCGGA T^CCGGA
2340 TCCGGA
BsrDI 1331 GCAATG GCAATG(2/0)
1976 GCAATG
BstAPI 2689 GCAGCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2787 GCAATTCATGC
Bsu36I 1350 CCTGAGG CC^T[ACGT]AGG
1843 CCTGAGG
CelII 196 GCTGAGC GC^T[ACGT]AGC
387 GCTCAGC
DrdI 366 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2484 GACGGAGTCGTC
EagI 61 CGGCCG C^GGCCG
2569 CGGCCG
EclXI 61 CGGCCG C^GGCCG
2569 CGGCCG
Eco52I 61 CGGCCG C^GGCCG
2569 CGGCCG
Eco81I 1350 CCTGAGG CC^T[ACGT]AGG
1843 CCTGAGG
KpnI 1510 GGTACC GGTAC^C
2740 GGTACC
MaeI 2191 CTAG C^TAG
2447 CTAG
MaeII 443 ACGT A^CGT
1829 ACGT
MroI 761 TCCGGA T^CCGGA
2340 TCCGGA
MseI 619 TTAA T^TAA
901 TTAA
NcoI 1028 CCATGG C^CATGG
1983 CCATGG
PflMI 551 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCACGAGATGG
PvuII 1121 CAGCTG CAG^CTG
1582 CAGCTG
SacI 199 GAGCTC GAGCT^C
1737 GAGCTC
SmaI 513 CCCGGG CCC^GGG
2464 CCCGGG
Tru9I 619 TTAA T^TAA
901 TTAA
Van91I 551 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCACGAGATGG
XmaCI 513 CCCGGG C^CCGGG
2464 CCCGGG
XmaI 513 CCCGGG C^CCGGG
2464 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII