Restriction map for
pF1KB7299
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# NO CUT ENZYMES ( 64 enzymes) #
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AccIII AclI AgeI AhdI AscI
AspEI AvrII BamHI BbeI BbrPI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DrdI Eam1105I
EcoRI EcoRV HindIII HpaI KasI
MamI MluI MroI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 2197 GACGTC GACGT^C
Acc65I 3597 GGTACC G^GTACC
AccI 892 GTCGAC GT^[AC][GT]AC
AcyI 2197 GACGTC G[GA]^CG[CT]C
AflII 1083 CTTAAG C^TTAAG
ApaI 2977 GGGCCC GGGCC^C
AseI 1255 ATTAAT AT^TAAT
AsnI 1255 ATTAAT AT^TAAT
Asp700I 3216 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3597 GGTACC G^GTACC
AviII 2363 TGCGCA TGC^GCA
BbvCI 2911 CCTCAGC CCTCAGC(-5/-2)
BclI 1476 TGATCA T^GATCA
BfrI 1083 CTTAAG C^TTAAG
BsaHI 2197 GACGTC G[GA]^CG[CT]C
BsmBI 2340 CGTCTC CGTCTC(1/5)
Bsp1407I 611 TGTACA T^GTACA
BsrGI 611 TGTACA T^GTACA
DraI 1597 TTTAAA TTT^AAA
DraIII 1545 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2924 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3112 ATGCAT ATGCA^T
FbaI 1476 TGATCA T^GATCA
FspI 2363 TGCGCA TGC^GCA
Hin1I 2197 GACGTC G[GA]^CG[CT]C
KpnI 3597 GGTACC GGTAC^C
KspI 143 CCGCGG CCGC^GG
MfeI 1013 CAATTG C^AATTG
MunI 1013 CAATTG C^AATTG
NsiI 3112 ATGCAT ATGCA^T
PaeR7I 3375 CTCGAG C^TCGAG
PflMI 1852 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1255 ATTAAT AT^TAAT
SacII 143 CCGCGG CCGC^GG
SalI 892 GTCGAC G^TCGAC
SapI 257 GAAGAGC GCTCTTC(1/4)
SfiI 3510 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1586 GCATGC GCATG^C
SspBI 611 TGTACA T^GTACA
StuI 2936 AGGCCT AGG^CCT
Van91I 1852 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1032 TCTAGA T^CTAGA
XhoI 3375 CTCGAG C^TCGAG
XmnI 3216 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 537 CCTAAGG CC^T[ACGT]AGG
3479 CCTCAGG
Aor51HI 833 AGCGCT AGC^GCT
3134 AGCGCT
BalI 918 TGGCCA TGG^CCA
1758 TGGCCA
BcgI 3443 CGAGGTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2438 GCACTACTTTCG
BciVI 1506 GTATCC GTATCC(6/5)
2318 GTATCC
BfaI 352 CTAG C^TAG
1033 CTAG
BglI 64 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3511 GCCTCCCTGGC
BseRI 134 CTCCTC GAGGAG(10/8)
468 CTCCTC
BspHI 1137 TCATGA T^CATGA
1326 TCATGA
BsrBI 1454 CCGCTC CCGCTC(-3/-3)
148 GAGCGG
BssHII 11 GCGCGC G^CGCGC
13 GCGCGC
Bsu36I 537 CCTAAGG CC^T[ACGT]AGG
3479 CCTCAGG
Eco47III 833 AGCGCT AGC^GCT
3134 AGCGCT
Eco81I 537 CCTAAGG CC^T[ACGT]AGG
3479 CCTCAGG
FseI 3469 GGCCGGCC GGCCGG^CC
3473 GGCCGGCC
MaeI 352 CTAG C^TAG
1033 CTAG
MluNI 918 TGGCCA TGG^CCA
1758 TGGCCA
MscI 918 TGGCCA TGG^CCA
1758 TGGCCA
NaeI 3470 GCCGGC GCC^GGC
3474 GCCGGC
NgoMIV 3470 GCCGGC G^CCGGC
3474 GCCGGC
RcaI 1137 TCATGA T^CATGA
1326 TCATGA
SexAI 182 ACCTGGT A^CC[AT]GGT
3593 ACCTGGT
SmaI 1922 CCCGGG CCC^GGG
2544 CCCGGG
XmaCI 1922 CCCGGG C^CCGGG
2544 CCCGGG
XmaI 1922 CCCGGG C^CCGGG
2544 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII