Restriction map for
pF1KB7296
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AviII
BalI BbeI BbvCI BcgI BciVI
BclI BcnI BfrI BpmI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FbaI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KspI MluI MluNI
MroI MscI NaeI NarI NciI
NgoMIV NotI NruI NspV PacI
PflMI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI Sse8387I StuI SwaI
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 3142 GGTACC G^GTACC
AccII 45 CGCG CG^CG
Asp718I 3142 GGTACC G^GTACC
AspI 2240 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3200 GGATCC G^GATCC
BbrPI 1352 CACGTG CAC^GTG
BglI 48 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 184 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 184 GCTCAGC GC^T[ACGT]AGC
BsmI 3984 GAATGC GAATGC(1/-1)
Bsp1407I 2394 TGTACA T^GTACA
BspMI 2324 ACCTGC ACCTGC(4/8)
BsrGI 2394 TGTACA T^GTACA
BssSI 3647 CACGAG CACGAG(-5/-1)
BstUI 45 CGCG CG^CG
CelII 184 GCTCAGC GC^T[ACGT]AGC
EcoT22I 2120 ATGCAT ATGCA^T
FauI 57 GCGGG CCCGC(4/6)
HincII 3258 GTTAAC GT[CT]^[GA]AC
HindII 3258 GTTAAC GT[CT]^[GA]AC
HpaI 3258 GTTAAC GTT^AAC
KpnI 3142 GGTACC GGTAC^C
MvnI 45 CGCG CG^CG
NheI 1447 GCTAGC G^CTAGC
NsiI 2120 ATGCAT ATGCA^T
PflFI 2240 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1352 CACGTG CAC^GTG
PmlI 1352 CACGTG CAC^GTG
SnaBI 1839 TACGTA TAC^GTA
SpeI 831 ACTAGT A^CTAGT
SphI 1533 GCATGC GCATG^C
SspBI 2394 TGTACA T^GTACA
Tsp45I 3725 GTGAC ^GT[GC]AC
Tth111I 2240 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 41 enzymes) #
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AocI 584 CCTCAGG CC^T[ACGT]AGG
2833 CCTGAGG
AspHI 2092 GAGCAC G[AT]GC[AT]^C
3937 GAGCAC
AvaI 112 CTCGAG C^[CT]CG[GA]G
896 CTCGAG
AvrII 351 CCTAGG C^CTAGG
3550 CCTAGG
BanI 69 GGTGCC G^G[CT][GA]CC
3142 GGTACC
BanII 682 GAGCCC G[GA]GC[CT]^C
3330 GAGCCC
BglII 2379 AGATCT A^GATCT
3391 AGATCT
BlnI 351 CCTAGG C^CTAGG
3550 CCTAGG
BsaAI 1352 CACGTG [CT]AC^GT[GA]
1839 TACGTA
BsaBI 2119 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3316 GATTCCGATC
BsaWI 1637 ACCGGA [AT]^CCGG[AT]
3599 ACCGGA
BsgI 3233 CTGCAC GTGCAG(16/14)
2012 GTGCAG
BsiHKAI 2092 GAGCAC G[AT]GC[AT]^C
3937 GAGCAC
BsoBI 112 CTCGAG C^[CT]CG[GA]G
896 CTCGAG
BspHI 921 TCATGA T^CATGA
2712 TCATGA
BstAPI 9 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
493 GCAATGTGTGC
Bsu36I 584 CCTCAGG CC^T[ACGT]AGG
2833 CCTGAGG
DraII 347 AGGTCCT [GA]G^G[ACGT]CC[CT]
2837 AGGTCCT
DraIII 2493 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2771 CACCATGTG
DsaI 3381 CCATGG C^C[GA][CT]GG
3729 CCATGG
Eco81I 584 CCTCAGG CC^T[ACGT]AGG
2833 CCTGAGG
EcoO109I 347 AGGTCCT [GA]G^G[ACGT]CC[CT]
2837 AGGTCCT
EcoRI 1790 GAATTC G^AATTC
2634 GAATTC
EcoRV 1623 GATATC GAT^ATC
2956 GATATC
HapII 1638 CCGG C^CGG
3600 CCGG
HpaII 1638 CCGG C^CGG
3600 CCGG
MamI 2119 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3316 GATTCCGATC
MfeI 242 CAATTG C^AATTG
1605 CAATTG
MspA1I 13 CAGCTG C[AC]G^C[GT]G
1589 CAGCTG
MspI 1638 CCGG C^CGG
3600 CCGG
MunI 242 CAATTG C^AATTG
1605 CAATTG
NcoI 3381 CCATGG C^CATGG
3729 CCATGG
NdeI 881 CATATG CA^TATG
1846 CATATG
PaeR7I 112 CTCGAG C^TCGAG
896 CTCGAG
PpuMI 347 AGGTCCT [GA]G^G[AT]CC[CT]
2837 AGGTCCT
PstI 413 CTGCAG CTGCA^G
3166 CTGCAG
PvuII 13 CAGCTG CAG^CTG
1589 CAGCTG
RcaI 921 TCATGA T^CATGA
2712 TCATGA
SapI 571 GAAGAGC GCTCTTC(1/4)
130 GCTCTTC
XcmI 241 CCAATTGATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1019 CCAGGCTGCTTCTGG
XhoI 112 CTCGAG C^TCGAG
896 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BfaI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI