Restriction map for
pF1KB7289
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AccI AflII AgeI
AhdI Alw44I ApaLI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BfrI BglII BlnI BsiWI BsmBI
BsmI Bsp1407I BspDI BsrGI BssSI
Bst1107I BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoT22I FbaI FspI
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI NarI NcoI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RsrII
SacII SalI SexAI SfiI SfoI
SgrAI SmlI SnaBI SphI SspBI
SspI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AclI 1363 AACGTT AA^CGTT
AcsI 742 AAATTC [GA]^AATT[CT]
AcyI 2575 GGCGTC G[GA]^CG[CT]C
AflIII 2387 ACATGT A^C[GA][CT]GT
AocI 2728 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1590 AGCGCT AGC^GCT
ApoI 742 AAATTC [GA]^AATT[CT]
AscI 2095 GGCGCGCC GG^CGCGCC
AspHI 2333 GAGCTC G[AT]GC[AT]^C
AspI 406 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 443 GGTCC G^G[AT]CC
BsaHI 2575 GGCGTC G[GA]^CG[CT]C
BsaI 2782 GAGACC GGTCTC(1/5)
BsiEI 1960 CGGTCG CG[GA][CT]^CG
BsiHKAI 2333 GAGCTC G[AT]GC[AT]^C
BspHI 1394 TCATGA T^CATGA
BspLU11I 2387 ACATGT A^CATGT
BsrBI 1957 GAGCGG CCGCTC(-3/-3)
BsrFI 2108 GCCGGC [GA]^CCGG[CT]
BstAPI 1164 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1639 TTCGAA TT^CGAA
Bsu36I 2728 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2108 GCCGGC [GA]^CCGG[CT]
Eco47III 1590 AGCGCT AGC^GCT
Eco81I 2728 CCTGAGG CC^T[ACGT]AGG
EcoRV 793 GATATC GAT^ATC
FseI 2107 GGCCGGCC GGCCGG^CC
HgaI 2576 GCGTC GACGC(5/10)
Hin1I 2575 GGCGTC G[GA]^CG[CT]C
MseI 1645 TTAA T^TAA
NaeI 2108 GCCGGC GCC^GGC
NdeI 1862 CATATG CA^TATG
NgoMIV 2108 GCCGGC G^CCGGC
NspI 2387 ACATGT [GA]CATG^[CT]
NspV 1639 TTCGAA TT^CGAA
PflFI 406 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1363 AACGTT AA^CGTT
RcaI 1394 TCATGA T^CATGA
SacI 2333 GAGCTC GAGCT^C
SapI 2224 GAAGAGC GCTCTTC(1/4)
SbfI 2278 CCTGCAGG CCTGCA^GG
ScaI 1273 AGTACT AGT^ACT
SfuI 1639 TTCGAA TT^CGAA
SmaI 1355 CCCGGG CCC^GGG
SpeI 504 ACTAGT A^CTAGT
Sse8387I 2278 CCTGCAGG CCTGCA^GG
Tru9I 1645 TTAA T^TAA
Tth111I 406 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1355 CCCGGG C^CCGGG
XmaI 1355 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 137 TCCGGA T^CCGGA
2660 TCCGGA
ApaI 696 GGGCCC GGGCC^C
2913 GGGCCC
AvaI 1355 CCCGGG C^[CT]CG[GA]G
2113 CCCGAG
BanI 1600 GGTGCC G^G[CT][GA]CC
2694 GGCACC
BbvCI 735 CCTCAGC CCTCAGC(-5/-2)
1143 CCTCAGC
BciVI 305 GTATCC GTATCC(6/5)
1176 GTATCC
BfaI 505 CTAG C^TAG
1468 CTAG
BglI 631 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2229 GCCCAATGGGC
BsaAI 1829 TACGTG [CT]AC^GT[GA]
2548 TACGTG
BsaBI 274 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1407 GATGTCCATC
BsaWI 137 TCCGGA [AT]^CCGG[AT]
2660 TCCGGA
BseAI 137 TCCGGA T^CCGGA
2660 TCCGGA
BsoBI 1355 CCCGGG C^[CT]CG[GA]G
2113 CCCGAG
BspEI 137 TCCGGA T^CCGGA
2660 TCCGGA
BspMI 2723 ACCTGC ACCTGC(4/8)
2281 GCAGGT
BsrDI 711 CATTGC GCAATG(2/0)
1200 CATTGC
BssHII 2013 GCGCGC G^CGCGC
2096 GCGCGC
BstEII 492 GGTCACC G^GT[ACGT]ACC
873 GGTCACC
BstPI 492 GGTCACC G^GT[ACGT]ACC
873 GGTCACC
BstYI 353 GGATCT [GA]^GATC[CT]
1553 AGATCC
DsaI 260 CCGTGG C^C[GA][CT]GG
683 CCGTGG
EcoNI 315 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2724 CCTGCCTGAGG
EcoO65I 492 GGTCACC G^GT[ACGT]ACC
873 GGTCACC
HincII 1360 GTCAAC GT[CT]^[GA]AC
1918 GTTGAC
HindII 1360 GTCAAC GT[CT]^[GA]AC
1918 GTTGAC
MaeI 505 CTAG C^TAG
1468 CTAG
MamI 274 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1407 GATGTCCATC
MflI 353 GGATCT [GA]^GATC[CT]
1553 AGATCC
MroI 137 TCCGGA T^CCGGA
2660 TCCGGA
XhoII 353 GGATCT [GA]^GATC[CT]
1553 AGATCC
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BalI BanII BbsI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI