Restriction map for
pF1KB7273
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AgeI Alw44I Aor51HI
ApaI ApaLI AscI AspI AvaI
AviII BamHI BbrPI BbvCI BcgI
BcnI BglII BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspEI
BsrBI BsrFI BssHII BssSI BstBI
Cfr10I CpoI DraII DrdI EagI
EclXI Eco47III Eco52I EcoO109I FseI
FspI KspI MluI MroI NaeI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AatII 1184 GACGTC GACGT^C
Acc65I 3807 GGTACC G^GTACC
AccII 3812 CGCG CG^CG
AflII 2749 CTTAAG C^TTAAG
AhdI 62 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3143 CCTCAGG CC^T[ACGT]AGG
AseI 1818 ATTAAT AT^TAAT
AsnI 1818 ATTAAT AT^TAAT
Asp718I 3807 GGTACC G^GTACC
AspEI 62 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3076 CCTAGG C^CTAGG
BanII 3424 GAGCCC G[GA]GC[CT]^C
BbeI 9 GGCGCC GGCGC^C
BfrI 2749 CTTAAG C^TTAAG
BglI 3128 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3076 CCTAGG C^CTAGG
BlpI 1914 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1914 GCTGAGC GC^T[ACGT]AGC
BsaAI 3759 TACGTG [CT]AC^GT[GA]
BsaI 69 GGTCTC GGTCTC(1/5)
Bsp1407I 3956 TGTACA T^GTACA
BspDI 1279 ATCGAT AT^CGAT
BspLU11I 521 ACATGT A^CATGT
BspMI 945 ACCTGC ACCTGC(4/8)
BsrGI 3956 TGTACA T^GTACA
Bst1107I 2641 GTATAC GTA^TAC
BstAPI 268 GCACAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3160 GGTCACC G^GT[ACGT]ACC
BstPI 3160 GGTCACC G^GT[ACGT]ACC
BstUI 3812 CGCG CG^CG
BstXI 2272 CCAATTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2641 GTATAC GTA^TAC
Bsu36I 3143 CCTCAGG CC^T[ACGT]AGG
CelII 1914 GCTGAGC GC^T[ACGT]AGC
CfoI 10 GCGC GCG^C
ClaI 1279 ATCGAT AT^CGAT
DraIII 2765 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2981 CCATGG C^C[GA][CT]GG
Eam1105I 62 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3143 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3160 GGTCACC G^GT[ACGT]ACC
EcoRV 715 GATATC GAT^ATC
FauI 57 GCGGG CCCGC(4/6)
HaeII 9 GGCGCC [GA]GCGC^[CT]
HapII 456 CCGG C^CGG
HhaI 10 GCGC GCG^C
HinP1I 10 GCGC G^CGC
HindIII 3198 AAGCTT A^AGCTT
HpaI 1830 GTTAAC GTT^AAC
HpaII 456 CCGG C^CGG
KasI 9 GGCGCC G^GCGCC
KpnI 3807 GGTACC GGTAC^C
MspI 456 CCGG C^CGG
MvnI 3812 CGCG CG^CG
NarI 9 GGCGCC GG^CGCC
NcoI 2981 CCATGG C^CATGG
NdeI 1840 CATATG CA^TATG
PshBI 1818 ATTAAT AT^TAAT
SalI 1187 GTCGAC G^TCGAC
SfoI 9 GGCGCC GGC^GCC
SmlI 2749 CTTAAG C^T[CT][GA]AG
SphI 1527 GCATGC GCATG^C
SspBI 3956 TGTACA T^GTACA
StuI 3115 AGGCCT AGG^CCT
XbaI 1641 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1187 GTCGAC GT^[AC][GT]AC
2641 GTATAC
AcyI 9 GGCGCC G[GA]^CG[CT]C
1184 GACGTC
AflIII 521 ACATGT A^C[GA][CT]GT
3760 ACGTGT
AlwNI 3278 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
3327 CAGCTCCTG
Asp700I 676 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2589 GAATAATTTC
AspHI 1519 GAGCAC G[AT]GC[AT]^C
3826 GTGCTC
BalI 938 TGGCCA TGG^CCA
1419 TGGCCA
BciVI 376 GTATCC GTATCC(6/5)
1543 GTATCC
BsaBI 3313 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3505 GATGATGATC
BsaHI 9 GGCGCC G[GA]^CG[CT]C
1184 GACGTC
BsiHKAI 1519 GAGCAC G[AT]GC[AT]^C
3826 GTGCTC
BsmFI 60 GGGAC GGGAC(10/14)
1335 GGGAC
BsrDI 258 CATTGC GCAATG(2/0)
972 CATTGC
EaeI 938 TGGCCA [CT]^GGCC[GA]
1419 TGGCCA
EcoRI 1321 GAATTC G^AATTC
3888 GAATTC
HgaI 1963 GACGC GACGC(5/10)
3813 GCGTC
Hin1I 9 GGCGCC G[GA]^CG[CT]C
1184 GACGTC
HincII 1187 GTCGAC GT[CT]^[GA]AC
1830 GTTAAC
HindII 1187 GTCGAC GT[CT]^[GA]AC
1830 GTTAAC
MamI 3313 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3505 GATGATGATC
MluNI 938 TGGCCA TGG^CCA
1419 TGGCCA
MscI 938 TGGCCA TGG^CCA
1419 TGGCCA
PstI 947 CTGCAG CTGCA^G
2671 CTGCAG
ScaI 1636 AGTACT AGT^ACT
2544 AGTACT
XcmI 241 CCAATTGATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2269 CCACCAATTGAATGG
XmnI 676 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2589 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoNI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII