Restriction map for
pF1KB7212
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I TseI Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 262 GTCTAC GT^[AC][GT]AC
AfaI 64 GTAC GT^AC
AlwI 198 GATCC GGATC(4/5)
Asp700I 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 223 GAGCAC G[AT]GC[AT]^C
BanI 107 GGCGCC G^G[CT][GA]CC
BbeI 107 GGCGCC GGCGC^C
BcnI 201 CCGGG CC^[GC]GG
BglI 119 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 46 GCTAAGC GC^T[ACGT]AGC
BmyI 223 GAGCAC G[AGT]GC[ACT]^C
BpmI 187 CTGGAG CTGGAG(16/14)
Bpu1102I 46 GCTAAGC GC^T[ACGT]AGC
BseRI 157 GAGGAG GAGGAG(10/8)
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
BsmAI 160 GAGAC GTCTC(1/5)
BsmFI 203 GGGAC GGGAC(10/14)
Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C
BsrFI 96 GCCGGC [GA]^CCGG[CT]
BstEII 243 GGTCACC G^GT[ACGT]ACC
BstPI 243 GGTCACC G^GT[ACGT]ACC
BstXI 251 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 46 GCTAAGC GC^T[ACGT]AGC
Cfr10I 96 GCCGGC [GA]^CCGG[CT]
Cfr13I 282 GGGCC G^G[ACGT]CC
DdeI 47 CTAAG C^T[ACGT]AG
DpnII 198 GATC ^GATC
EcoO65I 243 GGTCACC G^GT[ACGT]ACC
EcoT14I 251 CCATGG C^C[AT][AT]GG
HgaI 205 GACGC GACGC(5/10)
HinfI 162 GACTC G^A[ACGT]TC
KasI 107 GGCGCC G^GCGCC
MaeII 194 ACGT A^CGT
MboI 198 GATC ^GATC
NaeI 96 GCCGGC GCC^GGC
NarI 107 GGCGCC GG^CGCC
NciI 201 CCGGG CC^[GC]GG
NcoI 251 CCATGG C^CATGG
NdeII 198 GATC ^GATC
NgoMIV 96 GCCGGC G^CCGGC
NlaIII 252 CATG CATG^
NlaIV 107 GGCGCC GG[ACGT]^[ACGT]CC
PleI 162 GACTC GAGTC(4/5)
RsaI 64 GTAC GT^AC
Sau3AI 198 GATC ^GATC
Sau96I 282 GGGCC G^G[ACGT]CC
ScaI 63 AGTACT AGT^ACT
SfoI 107 GGCGCC GGC^GCC
SmlI 271 CTCAAG C^T[CT][GA]AG
StyI 251 CCATGG C^C[AT][AT]GG
XmnI 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcyI 107 GGCGCC G[GA]^CG[CT]C
205 GACGCC
BsaHI 107 GGCGCC G[GA]^CG[CT]C
205 GACGCC
BstF5I 102 CATCC GGATG(2/0)
255 GGATG
EaeI 6 TGGCCG [CT]^GGCC[GA]
98 CGGCCA
FauI 20 GCGGG CCCGC(4/6)
167 GCGGG
FokI 102 CATCC GGATG(9/13)
255 GGATG
Hin1I 107 GGCGCC G[GA]^CG[CT]C
205 GACGCC
HphI 89 TCACC GGTGA(8/7)
245 TCACC
KspI 9 CCGCGG CCGC^GG
120 CCGCGG
MaeIII 211 GTGAC ^GT[ACGT]AC
244 GTCAC
MslI 246 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CATGGATGTG
MspA1I 9 CCGCGG C[AC]G^C[GT]G
120 CCGCGG
SacII 9 CCGCGG CCGC^GG
120 CCGCGG
SfaNI 86 GCATC GCATC(5/9)
137 GCATC
Tsp45I 211 GTGAC ^GT[GC]AC
244 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AccII AciI BsaJI BssKI BstNI
BstUI Cac8I CfoI DsaI EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MvnI MwoI ScrFI