##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AfaI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstYI       
Bsu36I      CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          272  GTATAC               GT^[AC][GT]AC
AccII         352  CGCG                 CG^CG
AciI           44  CCGC                 CCGC(-3/-1)
AflII         193  CTTAAG               C^TTAAG
AluI          137  AGCT                 AG^CT
BbvI           62  GCAGC                GCAGC(8/12)
BfaI           59  CTAG                 C^TAG
BfrI          193  CTTAAG               C^TTAAG
BpmI          112  CTGGAG               CTGGAG(16/14)
BsmAI         115  GAGAC                GTCTC(1/5)
BspMI          39  ACCTGC               ACCTGC(4/8)
BssKI         268  CCAGG                ^CC[ACGT]GG
Bst1107I      272  GTATAC               GTA^TAC
BstNI         268  CCAGG                CC^[AT]GG
BstUI         352  CGCG                 CG^CG
BstZ17I       272  GTATAC               GTA^TAC
Cac8I          58  GCTAGC               GC[ACGT]^[ACGT]GC
CfoI          351  GCGC                 GCG^C
DpnII         109  GATC                 ^GATC
DraI          334  TTTAAA               TTT^AAA
EcoRII        268  CCAGG                ^CC[AT]GG
HhaI          351  GCGC                 GCG^C
HinP1I        351  GCGC                 G^CGC
MaeI           59  CTAG                 C^TAG
MaeII         316  ACGT                 A^CGT
MaeIII        253  GTCAC                ^GT[ACGT]AC
MboI          109  GATC                 ^GATC
MnlI           26  CCTC                 CCTC(7/6)
MvaI          268  CCAGG                CC^[AT]GG
MvnI          352  CGCG                 CG^CG
NdeII         109  GATC                 ^GATC
NheI           58  GCTAGC               G^CTAGC
Sau3AI        109  GATC                 ^GATC
ScrFI         268  CCAGG                CC^[ACGT]GG
SmlI          193  CTTAAG               C^T[CT][GA]AG
TseI           62  GCAGC                G^C[AT]GC
Tsp45I        253  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AcsI          330  AAATTT               [GA]^AATT[CT]
              337  AAATTT              
ApoI          330  AAATTT               [GA]^AATT[CT]
              337  AAATTT              
DdeI           93  CTTAG                C^T[ACGT]AG
              230  CTGAG               
Fnu4HI         43  GCCGC                GC^[ACGT]GC
               62  GCAGC               
ItaI           43  GCCGC                GC^[ACGT]GC
               62  GCAGC               
MseI          194  TTAA                 T^TAA
              335  TTAA                
Tru9I         194  TTAA                 T^TAA
              335  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

Tsp509I