##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstYI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau3AI      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        TfiI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccI          280  GTATAC               GT^[AC][GT]AC
AciI          545  CCGC                 CCGC(-3/-1)
AseI          598  ATTAAT               AT^TAAT
AsnI          598  ATTAAT               AT^TAAT
BbsI          311  GAAGAC               GAAGAC(2/6)
BbvI           28  GCTGC                GCAGC(8/12)
BciVI         296  GGATAC               GTATCC(6/5)
BpuAI         311  GAAGAC               GAAGAC(2/6)
BsmI          209  GAATGC               GAATGC(1/-1)
BsrI          133  CCAGT                ACTGG(1/-1)
Bst1107I      280  GTATAC               GTA^TAC
BstF5I        654  GGATG                GGATG(2/0)
BstZ17I       280  GTATAC               GTA^TAC
CfoI          632  GCGC                 GCG^C
Cfr13I        358  GGCCC                G^G[ACGT]CC
FokI          654  GGATG                GGATG(9/13)
HaeIII        358  GGCC                 GG^CC
HgaI          382  GACGC                GACGC(5/10)
HhaI          632  GCGC                 GCG^C
HinP1I        632  GCGC                 G^CGC
MaeII          24  ACGT                 A^CGT
MaeIII         78  GTTAC                ^GT[ACGT]AC
MwoI          376  GCAAAAGACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI         598  ATTAAT               AT^TAAT
SapI          571  GAAGAGC              GCTCTTC(1/4)
Sau96I        358  GGCCC                G^G[ACGT]CC
SspI           43  AATATT               AAT^ATT
TseI           28  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

Fnu4HI         28  GCTGC                GC^[ACGT]GC
              544  GCCGC               
ItaI           28  GCTGC                GC^[ACGT]GC
              544  GCCGC               
MseI           41  TTAA                 T^TAA
              599  TTAA                
NlaIII        273  CATG                 CATG^
              651  CATG                
SfaNI         330  GATGC                GCATC(5/9)
              541  GATGC               
Tru9I          41  TTAA                 T^TAA
              599  TTAA                
Tsp509I       440  AATT                 ^AATT
              601  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

Cac8I       EarI        HphI        Ksp632I     MboII       
MnlI        TspRI