##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbvCI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FspI HindIII HpaI KspI MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI MvnI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 260 GGTACC G^GTACC
AcsI 1234 GAATTT [GA]^AATT[CT]
AflIII 296 ACGTGT A^C[GA][CT]GT
AgeI 302 ACCGGT A^CCGGT
ApoI 1234 GAATTT [GA]^AATT[CT]
Asp718I 260 GGTACC G^GTACC
BamHI 156 GGATCC G^GATCC
BbeI 7 GGCGCC GGCGC^C
BbrPI 295 CACGTG CAC^GTG
BbsI 580 GTCTTC GAAGAC(2/6)
BcgI 127 CGACGCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 994 GTATCC GTATCC(6/5)
BpuAI 580 GTCTTC GAAGAC(2/6)
BsmAI 100 GAGAC GTCTC(1/5)
BsmBI 100 GAGACG CGTCTC(1/5)
BspHI 1086 TCATGA T^CATGA
BsrDI 786 GCAATG GCAATG(2/0)
BstXI 636 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1129 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1340 CCACGG C^C[GA][CT]GG
EarI 1242 GAAGAG CTCTTC(1/4)
EcoO109I 1129 GGGACCC [GA]G^G[ACGT]CC[CT]
FseI 60 GGCCGGCC GGCCGG^CC
HincII 1213 GTCAAC GT[CT]^[GA]AC
HindII 1213 GTCAAC GT[CT]^[GA]AC
KasI 7 GGCGCC G^GCGCC
KpnI 260 GGTACC GGTAC^C
Ksp632I 1242 GAAGAG CTCTTC(1/4)
MslI 948 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 7 GGCGCC GG^CGCC
NspI 1355 GCATGT [GA]CATG^[CT]
PinAI 302 ACCGGT A^CCGGT
PmaCI 295 CACGTG CAC^GTG
PmlI 295 CACGTG CAC^GTG
PpuMI 1129 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1086 TCATGA T^CATGA
SacI 1159 GAGCTC GAGCT^C
SapI 1242 GAAGAGC GCTCTTC(1/4)
ScaI 701 AGTACT AGT^ACT
SfoI 7 GGCGCC GGC^GCC
SmaI 1124 CCCGGG CCC^GGG
TaqI 197 TCGA T^CGA
TthHB8I 197 TCGA T^CGA
XmaCI 1124 CCCGGG C^CCGGG
XmaI 1124 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 7 GGCGCC G[GA]^CG[CT]C
128 GACGCC
AlwNI 418 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1180 CAGGGGCTG
Aor51HI 140 AGCGCT AGC^GCT
1253 AGCGCT
AvaI 43 CTCGGG C^[CT]CG[GA]G
1124 CCCGGG
BalI 214 TGGCCA TGG^CCA
1193 TGGCCA
BanII 646 GGGCTC G[GA]GC[CT]^C
1159 GAGCTC
BpmI 345 CTCCAG CTGGAG(16/14)
357 CTGGAG
BsaAI 295 CACGTG [CT]AC^GT[GA]
805 TACGTG
BsaHI 7 GGCGCC G[GA]^CG[CT]C
128 GACGCC
BsaWI 188 ACCGGA [AT]^CCGG[AT]
302 ACCGGT
BsoBI 43 CTCGGG C^[CT]CG[GA]G
1124 CCCGGG
Bsp1407I 752 TGTACA T^GTACA
1358 TGTACA
BspMI 263 ACCTGC ACCTGC(4/8)
512 ACCTGC
BsrGI 752 TGTACA T^GTACA
1358 TGTACA
BstYI 156 GGATCC [GA]^GATC[CT]
791 GGATCT
Eco47III 140 AGCGCT AGC^GCT
1253 AGCGCT
EcoNI 147 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
867 CCTCTATGAGG
HgaI 128 GACGC GACGC(5/10)
592 GACGC
Hin1I 7 GGCGCC G[GA]^CG[CT]C
128 GACGCC
HphI 954 GGTGA GGTGA(8/7)
1169 TCACC
MaeII 296 ACGT A^CGT
806 ACGT
MflI 156 GGATCC [GA]^GATC[CT]
791 GGATCT
MluNI 214 TGGCCA TGG^CCA
1193 TGGCCA
MscI 214 TGGCCA TGG^CCA
1193 TGGCCA
NaeI 4 GCCGGC GCC^GGC
61 GCCGGC
NgoMIV 4 GCCGGC G^CCGGC
61 GCCGGC
PstI 331 CTGCAG CTGCA^G
1383 CTGCAG
SfaNI 553 GCATC GCATC(5/9)
986 GCATC
SfcI 331 CTGCAG C^T[GA][CT]AG
1383 CTGCAG
SmlI 1264 CTTGAG C^T[CT][GA]AG
1306 CTCAAG
SspBI 752 TGTACA T^GTACA
1358 TGTACA
StuI 321 AGGCCT AGG^CCT
1376 AGGCCT
Tsp509I 718 AATT ^AATT
1235 AATT
XhoII 156 GGATCC [GA]^GATC[CT]
791 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BbvI BcnI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI TseI
TspRI