Restriction map for
pF1KB7187
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstXI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MscI MunI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacII SalI SbfI
ScaI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 1388 TCCGGA T^CCGGA
AcsI 1384 AAATTC [GA]^AATT[CT]
AlwI 1462 GATCC GGATC(4/5)
ApoI 1384 AAATTC [GA]^AATT[CT]
BbeI 228 GGCGCC GGCGC^C
BbsI 1598 GTCTTC GAAGAC(2/6)
BcgI 1001 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 669 AGATCT A^GATCT
BlpI 480 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 480 GCTGAGC GC^T[ACGT]AGC
BpuAI 1598 GTCTTC GAAGAC(2/6)
BsaWI 1388 TCCGGA [AT]^CCGG[AT]
BseAI 1388 TCCGGA T^CCGGA
BspEI 1388 TCCGGA T^CCGGA
BspHI 1310 TCATGA T^CATGA
BsrDI 1434 CATTGC GCAATG(2/0)
BssSI 391 CACGAG CACGAG(-5/-1)
BstAPI 1067 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 637 TTCGAA TT^CGAA
BstYI 669 AGATCT [GA]^GATC[CT]
CelII 480 GCTGAGC GC^T[ACGT]AGC
DraIII 1068 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1274 CCGTGG C^C[GA][CT]GG
EcoRV 616 GATATC GAT^ATC
HaeII 228 GGCGCC [GA]GCGC^[CT]
HgaI 217 GCGTC GACGC(5/10)
KasI 228 GGCGCC G^GCGCC
MflI 669 AGATCT [GA]^GATC[CT]
MroI 1388 TCCGGA T^CCGGA
MslI 1450 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 228 GGCGCC GG^CGCC
NspI 960 GCATGC [GA]CATG^[CT]
NspV 637 TTCGAA TT^CGAA
PpuMI 395 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1310 TCATGA T^CATGA
SacI 1402 GAGCTC GAGCT^C
SexAI 1163 ACCTGGT A^CC[AT]GGT
SfiI 78 GGCCTCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 228 GGCGCC GGC^GCC
SfuI 637 TTCGAA TT^CGAA
SphI 960 GCATGC GCATG^C
StuI 318 AGGCCT AGG^CCT
TfiI 784 GAATC G^A[AT]TC
XhoII 669 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcyI 216 GGCGTC G[GA]^CG[CT]C
228 GGCGCC
AlwNI 197 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
488 CAGCTTCTG
AocI 430 CCTGAGG CC^T[ACGT]AGG
1014 CCTCAGG
ApaI 21 GGGCCC GGGCC^C
928 GGGCCC
AspHI 1402 GAGCTC G[AT]GC[AT]^C
1469 GTGCTC
BsaHI 216 GGCGTC G[GA]^CG[CT]C
228 GGCGCC
BsaI 730 GAGACC GGTCTC(1/5)
875 GAGACC
BsiHKAI 1402 GAGCTC G[AT]GC[AT]^C
1469 GTGCTC
BsmAI 730 GAGAC GTCTC(1/5)
875 GAGAC
BspMI 1343 ACCTGC ACCTGC(4/8)
1190 GCAGGT
Bsu36I 430 CCTGAGG CC^T[ACGT]AGG
1014 CCTCAGG
DpnII 670 GATC ^GATC
1462 GATC
Eco81I 430 CCTGAGG CC^T[ACGT]AGG
1014 CCTCAGG
EcoNI 1184 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1506 CCTGAAAGAGG
EcoT14I 503 CCAAGG C^C[AT][AT]GG
515 CCTTGG
Hin1I 216 GGCGTC G[GA]^CG[CT]C
228 GGCGCC
MboI 670 GATC ^GATC
1462 GATC
MseI 1526 TTAA T^TAA
1612 TTAA
NaeI 93 GCCGGC GCC^GGC
187 GCCGGC
NdeII 670 GATC ^GATC
1462 GATC
NgoMIV 93 GCCGGC G^CCGGC
187 GCCGGC
PflMI 196 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
950 CCAGTCGGTGG
PstI 265 CTGCAG CTGCA^G
1188 CTGCAG
SapI 127 GAAGAGC GCTCTTC(1/4)
1305 GCTCTTC
Sau3AI 670 GATC ^GATC
1462 GATC
SmaI 24 CCCGGG CCC^GGG
931 CCCGGG
StyI 503 CCAAGG C^C[AT][AT]GG
515 CCTTGG
Tru9I 1526 TTAA T^TAA
1612 TTAA
Tsp509I 1385 AATT ^AATT
1609 AATT
Van91I 196 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
950 CCAGTCGGTGG
XmaCI 24 CCCGGG C^CCGGG
931 CCCGGG
XmaI 24 CCCGGG C^CCGGG
931 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoO65I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp45I TspRI TthHB8I