Restriction map for
pF1KB7185
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BpmI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 122 GGTACC G^GTACC
AccI 755 GTCGAC GT^[AC][GT]AC
AocI 556 CCTCAGG CC^T[ACGT]AGG
ApaI 503 GGGCCC GGGCC^C
Asp718I 122 GGTACC G^GTACC
AspI 402 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 824 CTCGAG C^[CT]CG[GA]G
BbsI 1009 GAAGAC GAAGAC(2/6)
BcgI 732 GCAAATATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 822 GCCTCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 596 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 596 GCTTAGC GC^T[ACGT]AGC
BpuAI 1009 GAAGAC GAAGAC(2/6)
BsaI 352 GAGACC GGTCTC(1/5)
BsoBI 824 CTCGAG C^[CT]CG[GA]G
BssSI 304 CTCGTG CACGAG(-5/-1)
BstAPI 1266 GCAAGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 261 GGTAACC G^GT[ACGT]ACC
BstPI 261 GGTAACC G^GT[ACGT]ACC
BstXI 563 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 556 CCTCAGG CC^T[ACGT]AGG
Cac8I 501 GCGGGC GC[ACGT]^[ACGT]GC
CelII 596 GCTTAGC GC^T[ACGT]AGC
DraII 164 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 556 CCTCAGG CC^T[ACGT]AGG
EcoO109I 164 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 261 GGTAACC G^GT[ACGT]ACC
EcoRI 112 GAATTC G^AATTC
EcoRV 343 GATATC GAT^ATC
FauI 501 GCGGG CCCGC(4/6)
HincII 755 GTCGAC GT[CT]^[GA]AC
HindII 755 GTCGAC GT[CT]^[GA]AC
KpnI 122 GGTACC GGTAC^C
MaeII 215 ACGT A^CGT
PaeR7I 824 CTCGAG C^TCGAG
PflFI 402 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 22 GACTC GAGTC(4/5)
PpuMI 164 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 805 CTGCAG CTGCA^G
SalI 755 GTCGAC G^TCGAC
SexAI 355 ACCTGGT A^CC[AT]GGT
SfaNI 962 GCATC GCATC(5/9)
SspI 735 AATATT AAT^ATT
Tth111I 402 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 824 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 635 AGCGCT AGC^GCT
859 AGCGCT
AvaII 165 GGTCC G^G[AT]CC
1315 GGACC
BanII 76 GAGCCC G[GA]GC[CT]^C
503 GGGCCC
BfaI 1196 CTAG C^TAG
1280 CTAG
BsgI 142 CTGCAC GTGCAG(16/14)
576 GTGCAG
BspMI 140 ACCTGC ACCTGC(4/8)
316 ACCTGC
BstYI 761 AGATCC [GA]^GATC[CT]
884 AGATCC
CfoI 636 GCGC GCG^C
860 GCGC
DraI 84 TTTAAA TTT^AAA
1123 TTTAAA
Eco47III 635 AGCGCT AGC^GCT
859 AGCGCT
EcoT14I 446 CCTTGG C^C[AT][AT]GG
1149 CCTTGG
HaeII 635 AGCGCT [GA]GCGC^[CT]
859 AGCGCT
HhaI 636 GCGC GCG^C
860 GCGC
HinP1I 636 GCGC G^CGC
860 GCGC
MaeI 1196 CTAG C^TAG
1280 CTAG
MboII 240 GAAGA GAAGA(8/7)
1009 GAAGA
MflI 761 AGATCC [GA]^GATC[CT]
884 AGATCC
SmlI 71 CTCAAG C^T[CT][GA]AG
824 CTCGAG
StyI 446 CCTTGG C^C[AT][AT]GG
1149 CCTTGG
XcmI 329 CCACACTGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1318 CCACTTCAAAACTGG
XhoII 761 AGATCC [GA]^GATC[CT]
884 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanI BbvI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I