##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        ItaI        KasI        
KpnI        KspI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MroI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TseI        Tsp45I      TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

BalI          206  TGGCCA               TGG^CCA
BanI          112  GGCACC               G^G[CT][GA]CC
BanII         352  GGGCTC               G[GA]GC[CT]^C
BbsI          442  GTCTTC               GAAGAC(2/6)
BcnI          600  CCGGG                CC^[GC]GG
BpmI          253  CTCCAG               CTGGAG(16/14)
BpuAI         442  GTCTTC               GAAGAC(2/6)
BseRI         259  GAGGAG               GAGGAG(10/8)
EaeI          206  TGGCCA               [CT]^GGCC[GA]
EarI          193  CTCTTC               CTCTTC(1/4)
HapII         600  CCGG                 C^CGG
HincII        199  GTCAAC               GT[CT]^[GA]AC
HindII        199  GTCAAC               GT[CT]^[GA]AC
HpaII         600  CCGG                 C^CGG
HphI          424  GGTGA                GGTGA(8/7)
Ksp632I       193  CTCTTC               CTCTTC(1/4)
MaeII         203  ACGT                 A^CGT
MluNI         206  TGGCCA               TGG^CCA
MscI          206  TGGCCA               TGG^CCA
MspI          600  CCGG                 C^CGG
MwoI          293  GCTTTCCCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          600  CCGGG                CC^[GC]GG
PpuMI         512  GGGACCC              [GA]G^G[AT]CC[CT]
SmlI          355  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          385  GTAC                 GT^AC
              639  GTAC                
Asp700I       438  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              645  GAAGCAATTC          
BmyI          352  GGGCTC               G[AGT]GC[ACT]^C
              489  GGGCAC              
Bsp1286I      352  GGGCTC               G[AGT]GC[ACT]^C
              489  GGGCAC              
Cac8I          46  GCCTGC               GC[ACGT]^[ACGT]GC
              182  GCAAGC              
CfoI          229  GCGC                 GCG^C
              588  GCGC                
DdeI          479  CTGAG                C^T[ACGT]AG
              615  CTCAG               
DraII         512  GGGACCC              [GA]G^G[ACGT]CC[CT]
              564  GGGGCCT             
EcoO109I      512  GGGACCC              [GA]G^G[ACGT]CC[CT]
              564  GGGGCCT             
HhaI          229  GCGC                 GCG^C
              588  GCGC                
HinP1I        229  GCGC                 G^CGC
              588  GCGC                
HinfI         342  GATTC                G^A[ACGT]TC
              575  GAATC               
MseI           13  TTAA                 T^TAA
              143  TTAA                
NlaIII        529  CATG                 CATG^
              585  CATG                
RsaI          385  GTAC                 GT^AC
              639  GTAC                
TfiI          342  GATTC                G^A[AT]TC
              575  GAATC               
Tru9I          13  TTAA                 T^TAA
              143  TTAA                
Tsp509I       308  AATT                 ^AATT
              650  AATT                
XmnI          438  GAAAGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              645  GAAGCAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        AvaII       BfaI        BsaJI       BsiYI       
BslI        BsmFI       BssKI       BstF5I      BstNI       
Cfr13I      EcoRII      FokI        HaeIII      MaeI        
MboII       MnlI        MvaI        NlaIV       Sau96I      
ScrFI