Restriction map for
pF1KB7181
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiWI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
CpoI DdeI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 823 GTCTAC GT^[AC][GT]AC
Alw44I 1051 GTGCAC G^TGCAC
AlwNI 121 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 304 GGGCCC GGGCC^C
ApaLI 1051 GTGCAC G^TGCAC
AspHI 1051 GTGCAC G[AT]GC[AT]^C
AspI 817 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 293 TGGCCA TGG^CCA
BbsI 651 GAAGAC GAAGAC(2/6)
BcgI 179 GCAATTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 361 GCCGGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 651 GAAGAC GAAGAC(2/6)
BsaI 769 GAGACC GGTCTC(1/5)
BsaWI 890 ACCGGA [AT]^CCGG[AT]
BseRI 25 CTCCTC GAGGAG(10/8)
BsgI 270 CTGCAC GTGCAG(16/14)
BsiEI 359 CGGCCG CG[GA][CT]^CG
BsiHKAI 1051 GTGCAC G[AT]GC[AT]^C
BsmBI 865 GAGACG CGTCTC(1/5)
BspDI 919 ATCGAT AT^CGAT
BsrBI 384 CCGCTC CCGCTC(-3/-3)
BsrDI 899 GCAATG GCAATG(2/0)
BsrFI 361 GCCGGC [GA]^CCGG[CT]
BssSI 960 CACGAG CACGAG(-5/-1)
BstXI 70 CCAATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 361 GCCGGC [GA]^CCGG[CT]
ClaI 919 ATCGAT AT^CGAT
EagI 359 CGGCCG C^GGCCG
EarI 793 CTCTTC CTCTTC(1/4)
EclXI 359 CGGCCG C^GGCCG
Eco52I 359 CGGCCG C^GGCCG
EcoNI 445 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 718 AGCGCC [GA]GCGC^[CT]
Ksp632I 793 CTCTTC CTCTTC(1/4)
MluNI 293 TGGCCA TGG^CCA
MscI 293 TGGCCA TGG^CCA
NaeI 361 GCCGGC GCC^GGC
NgoMIV 361 GCCGGC G^CCGGC
NspI 602 ACATGC [GA]CATG^[CT]
PflFI 817 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 471 CTGCAG CTGCA^G
SapI 792 GCTCTTC GCTCTTC(1/4)
ScaI 785 AGTACT AGT^ACT
SexAI 818 ACCTGGT A^CC[AT]GGT
SfcI 471 CTGCAG C^T[GA][CT]AG
SfiI 360 GGCCGGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 700 CTCAAG C^T[CT][GA]AG
StuI 147 AGGCCT AGG^CCT
Tth111I 817 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 102 GTAC GT^AC
786 GTAC
AvaI 16 CTCGGG C^[CT]CG[GA]G
755 CCCGAG
BanI 46 GGCACC G^G[CT][GA]CC
400 GGCACC
BsmAI 769 GAGAC GTCTC(1/5)
865 GAGAC
BsoBI 16 CTCGGG C^[CT]CG[GA]G
755 CCCGAG
BsrI 1004 ACTGG ACTGG(1/-1)
246 CCAGT
BssHII 523 GCGCGC G^CGCGC
544 GCGCGC
DpnII 192 GATC ^GATC
931 GATC
DraII 53 GGGTCCT [GA]G^G[ACGT]CC[CT]
815 GGGACCT
EcoO109I 53 GGGTCCT [GA]G^G[ACGT]CC[CT]
815 GGGACCT
HgaI 481 GACGC GACGC(5/10)
541 GACGC
MaeII 1016 ACGT A^CGT
1049 ACGT
MboI 192 GATC ^GATC
931 GATC
NdeII 192 GATC ^GATC
931 GATC
PpuMI 53 GGGTCCT [GA]G^G[AT]CC[CT]
815 GGGACCT
PvuII 639 CAGCTG CAG^CTG
1020 CAGCTG
RsaI 102 GTAC GT^AC
786 GTAC
Sau3AI 192 GATC ^GATC
931 GATC
Tsp509I 72 AATT ^AATT
181 AATT
TspRI 991 CACTG CA[GC]TG[ACGT][ACGT]^
1003 CACTG
XcmI 140 CCATCCCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
912 CCACGGCATCGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TthHB8I