##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MflI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 256 GTCTAC GT^[AC][GT]AC
AfaI 336 GTAC GT^AC
AflIII 358 ACGTGT A^C[GA][CT]GT
AspHI 1002 GAGCAC G[AT]GC[AT]^C
BbrPI 357 CACGTG CAC^GTG
BbsI 177 GAAGAC GAAGAC(2/6)
BbvI 459 GCTGC GCAGC(8/12)
BclI 626 TGATCA T^GATCA
BmyI 1002 GAGCAC G[AGT]GC[ACT]^C
BpmI 940 CTCCAG CTGGAG(16/14)
BpuAI 177 GAAGAC GAAGAC(2/6)
BsgI 1055 CTGCAC GTGCAG(16/14)
BsiHKAI 1002 GAGCAC G[AT]GC[AT]^C
BsmAI 362 GTCTC GTCTC(1/5)
Bsp1286I 1002 GAGCAC G[AGT]GC[ACT]^C
BspDI 374 ATCGAT AT^CGAT
BspHI 86 TCATGA T^CATGA
BspMI 442 ACCTGC ACCTGC(4/8)
ClaI 374 ATCGAT AT^CGAT
DraI 925 TTTAAA TTT^AAA
DraII 659 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 12 CTCTTC CTCTTC(1/4)
EcoNI 663 CCTAAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 659 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 407 CCCGC CCCGC(4/6)
FbaI 626 TGATCA T^GATCA
Fnu4HI 459 GCTGC GC^[ACGT]GC
ItaI 459 GCTGC GC^[ACGT]GC
Ksp632I 12 CTCTTC CTCTTC(1/4)
MspA1I 276 CCGCTG C[AC]G^C[GT]G
PleI 553 GAGTC GAGTC(4/5)
PmaCI 357 CACGTG CAC^GTG
PmlI 357 CACGTG CAC^GTG
RcaI 86 TCATGA T^CATGA
RsaI 336 GTAC GT^AC
SfcI 819 CTGTAG C^T[GA][CT]AG
TseI 459 GCTGC G^C[AT]GC
XcmI 593 CCAAAAATATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BalI 185 TGGCCA TGG^CCA
473 TGGCCA
BfaI 341 CTAG C^TAG
802 CTAG
BsaAI 357 CACGTG [CT]AC^GT[GA]
812 TACGTG
BsiYI 55 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
663 CCTAAAGAAGG
BslI 55 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
663 CCTAAAGAAGG
BssKI 381 CCTGG ^CC[ACGT]GG
755 CCTGG
BstF5I 1040 CATCC GGATG(2/0)
786 GGATG
BstNI 381 CCTGG CC^[AT]GG
755 CCTGG
DdeI 364 CTCAG C^T[ACGT]AG
1023 CTCAG
DpnII 373 GATC ^GATC
627 GATC
EcoRII 381 CCTGG ^CC[AT]GG
755 CCTGG
FokI 1040 CATCC GGATG(9/13)
786 GGATG
HgaI 416 GACGC GACGC(5/10)
320 GCGTC
MaeI 341 CTAG C^TAG
802 CTAG
MaeII 358 ACGT A^CGT
813 ACGT
MaeIII 439 GTCAC ^GT[ACGT]AC
815 GTGAC
MboI 373 GATC ^GATC
627 GATC
MfeI 731 CAATTG C^AATTG
882 CAATTG
MluNI 185 TGGCCA TGG^CCA
473 TGGCCA
MscI 185 TGGCCA TGG^CCA
473 TGGCCA
MunI 731 CAATTG C^AATTG
882 CAATTG
MvaI 381 CCTGG CC^[AT]GG
755 CCTGG
MwoI 410 GCCTTCGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
466 GCAGGCTTGGC
NdeII 373 GATC ^GATC
627 GATC
Sau3AI 373 GATC ^GATC
627 GATC
ScrFI 381 CCTGG CC^[ACGT]GG
755 CCTGG
SfaNI 446 GCATC GCATC(5/9)
806 GCATC
Tsp45I 439 GTCAC ^GT[GC]AC
815 GTGAC
TspRI 189 CAGTG CA[GC]TG[ACGT][ACGT]^
212 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AcsI AluI ApoI BsmFI
BsrI Cac8I Cfr13I EaeI HaeIII
HinfI HphI MboII MnlI MseI
MslI NlaIII Sau96I SspI TaqI
TfiI Tru9I Tsp509I TthHB8I