Restriction map for
pF1KB7129
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstUI BstXI BstYI
CelII CfoI Cfr10I CpoI DpnII
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaII KasI KpnI
KspI MamI MboI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp509I Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 408 CCGC CCGC(-3/-1)
AlwNI 232 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 867 CCTGAGG CC^T[ACGT]AGG
ApaI 252 GGGCCC GGGCC^C
AspHI 890 GAGCAC G[AT]GC[AT]^C
BanII 252 GGGCCC G[GA]GC[CT]^C
BbvCI 669 GCTGAGG CCTCAGC(-5/-2)
BfaI 907 CTAG C^TAG
BsaAI 361 TACGTG [CT]AC^GT[GA]
BsaI 817 GGTCTC GGTCTC(1/5)
BseRI 717 CTCCTC GAGGAG(10/8)
BsiHKAI 890 GAGCAC G[AT]GC[AT]^C
BspDI 459 ATCGAT AT^CGAT
BspMI 712 ACCTGC ACCTGC(4/8)
BsrDI 759 CATTGC GCAATG(2/0)
Bst1107I 859 GTATAC GTA^TAC
BstEII 735 GGTCACC G^GT[ACGT]ACC
BstPI 735 GGTCACC G^GT[ACGT]ACC
BstZ17I 859 GTATAC GTA^TAC
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
ClaI 459 ATCGAT AT^CGAT
DraIII 321 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 20 CCATGG C^C[GA][CT]GG
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
EcoO65I 735 GGTCACC G^GT[ACGT]ACC
FauI 407 CCCGC CCCGC(4/6)
HpaI 4 GTTAAC GTT^AAC
HphI 737 TCACC GGTGA(8/7)
MaeI 907 CTAG C^TAG
MaeII 362 ACGT A^CGT
MslI 606 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 421 CAGCTG C[AC]G^C[GT]G
MwoI 427 GCATCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 20 CCATGG C^CATGG
PvuII 421 CAGCTG CAG^CTG
SapI 294 GCTCTTC GCTCTTC(1/4)
SfcI 747 CTACAG C^T[GA][CT]AG
StuI 701 AGGCCT AGG^CCT
TfiI 546 GATTC G^A[AT]TC
Tsp45I 736 GTCAC ^GT[GC]AC
XcmI 266 CCATCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 766 GTCTAC GT^[AC][GT]AC
859 GTATAC
BbsI 261 GAAGAC GAAGAC(2/6)
85 GTCTTC
BbvI 420 GCAGC GCAGC(8/12)
943 GCTGC
BpuAI 261 GAAGAC GAAGAC(2/6)
85 GTCTTC
BsmAI 920 GAGAC GTCTC(1/5)
818 GTCTC
EcoT14I 20 CCATGG C^C[AT][AT]GG
795 CCAAGG
Fnu4HI 420 GCAGC GC^[ACGT]GC
943 GCTGC
HincII 4 GTTAAC GT[CT]^[GA]AC
241 GTCAAC
HindII 4 GTTAAC GT[CT]^[GA]AC
241 GTCAAC
ItaI 420 GCAGC GC^[ACGT]GC
943 GCTGC
MaeIII 736 GTCAC ^GT[ACGT]AC
903 GTTAC
NlaIII 21 CATG CATG^
692 CATG
PflMI 231 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
686 CCACAGCATGG
PpuMI 533 AGGTCCC [GA]G^G[AT]CC[CT]
709 GGGACCT
StyI 20 CCATGG C^C[AT][AT]GG
795 CCAAGG
TaqI 460 TCGA T^CGA
549 TCGA
TseI 420 GCAGC G^C[AT]GC
943 GCTGC
TthHB8I 460 TCGA T^CGA
549 TCGA
Van91I 231 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
686 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI AvaII BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII EarI EcoO109I
EcoRII FokI HaeIII HinfI Ksp632I
MboII MnlI MseI MvaI NlaIV
PleI RsaI Sau96I ScrFI SfaNI
Tru9I TspRI