Restriction map for
pF1KB7127
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FauI FbaI FokI
FseI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 808 GAATTC [GA]^AATT[CT]
AhdI 298 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 827 GGGCCC GGGCC^C
ApoI 808 GAATTC [GA]^AATT[CT]
AspEI 298 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 53 CCCGAG C^[CT]CG[GA]G
AviII 81 TGCGCA TGC^GCA
BalI 617 TGGCCA TGG^CCA
BamHI 720 GGATCC G^GATCC
BanI 156 GGTGCC G^G[CT][GA]CC
BanII 827 GGGCCC G[GA]GC[CT]^C
BbsI 703 GAAGAC GAAGAC(2/6)
BcnI 784 CCCGG CC^[GC]GG
BfaI 133 CTAG C^TAG
BpuAI 703 GAAGAC GAAGAC(2/6)
BsmFI 781 GTCCC GGGAC(10/14)
BsoBI 53 CCCGAG C^[CT]CG[GA]G
Bsp1407I 524 TGTACA T^GTACA
BsrGI 524 TGTACA T^GTACA
BsrI 464 CCAGT ACTGG(1/-1)
BstXI 536 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 82 GCGC GCG^C
Eam1105I 298 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 808 GAATTC G^AATTC
FspI 81 TGCGCA TGC^GCA
HhaI 82 GCGC GCG^C
HinP1I 82 GCGC G^CGC
HindIII 458 AAGCTT A^AGCTT
MaeI 133 CTAG C^TAG
MluNI 617 TGGCCA TGG^CCA
MscI 617 TGGCCA TGG^CCA
MslI 216 CACTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 475 CAGCTG C[AC]G^C[GT]G
NciI 784 CCCGG CC^[GC]GG
NdeI 865 CATATG CA^TATG
NspI 799 GCATGT [GA]CATG^[CT]
PvuII 475 CAGCTG CAG^CTG
SfaNI 488 GCATC GCATC(5/9)
SspBI 524 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 525 GTAC GT^AC
771 GTAC
AocI 193 CCTCAGG CC^T[ACGT]AGG
649 CCTGAGG
BmyI 644 GTGCCC G[AGT]GC[ACT]^C
827 GGGCCC
BsgI 859 CTGCAC GTGCAG(16/14)
751 GTGCAG
Bsp1286I 644 GTGCCC G[AGT]GC[ACT]^C
827 GGGCCC
BspMI 538 ACCTGC ACCTGC(4/8)
777 GCAGGT
BsrFI 384 GCCGGT [GA]^CCGG[CT]
773 ACCGGC
BstYI 177 AGATCC [GA]^GATC[CT]
720 GGATCC
Bsu36I 193 CCTCAGG CC^T[ACGT]AGG
649 CCTGAGG
Cfr10I 384 GCCGGT [GA]^CCGG[CT]
773 ACCGGC
Eco81I 193 CCTCAGG CC^T[ACGT]AGG
649 CCTGAGG
EcoNI 34 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
147 CCTGGGGGAGG
EcoT14I 590 CCAAGG C^C[AT][AT]GG
823 CCAAGG
MflI 177 AGATCC [GA]^GATC[CT]
720 GGATCC
PpuMI 779 AGGTCCC [GA]G^G[AT]CC[CT]
885 AGGACCT
RsaI 525 GTAC GT^AC
771 GTAC
StuI 42 AGGCCT AGG^CCT
597 AGGCCT
StyI 590 CCAAGG C^C[AT][AT]GG
823 CCAAGG
TaqI 372 TCGA T^CGA
491 TCGA
Tsp509I 424 AATT ^AATT
809 AATT
TthHB8I 372 TCGA T^CGA
491 TCGA
XhoII 177 AGATCC [GA]^GATC[CT]
720 GGATCC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AlwI AvaII BbvI BpmI
BsaJI BseRI BsiYI BslI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SmlI TseI Tsp45I
TspRI