Restriction map for
pF1KB7125
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MaeIII MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 22 GTCTAC GT^[AC][GT]AC
AcsI 129 AAATTC [GA]^AATT[CT]
AluI 422 AGCT AG^CT
ApoI 129 AAATTC [GA]^AATT[CT]
BamHI 53 GGATCC G^GATCC
BanII 175 GAGCCC G[GA]GC[CT]^C
BbsI 748 GTCTTC GAAGAC(2/6)
BmyI 175 GAGCCC G[AGT]GC[ACT]^C
BpmI 754 CTCCAG CTGGAG(16/14)
BpuAI 748 GTCTTC GAAGAC(2/6)
BsaBI 669 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 803 GGTCTC GGTCTC(1/5)
BsgI 73 GTGCAG GTGCAG(16/14)
BsmFI 856 GTCCC GGGAC(10/14)
Bsp1286I 175 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 182 TGTACA T^GTACA
BspMI 727 ACCTGC ACCTGC(4/8)
BsrFI 500 GCCGGT [GA]^CCGG[CT]
BsrGI 182 TGTACA T^GTACA
BsrI 756 CCAGT ACTGG(1/-1)
BstXI 356 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 53 GGATCC [GA]^GATC[CT]
CfoI 162 GCGC GCG^C
Cfr10I 500 GCCGGT [GA]^CCGG[CT]
DpnII 54 GATC ^GATC
DraI 712 TTTAAA TTT^AAA
DsaI 356 CCATGG C^C[GA][CT]GG
EcoT14I 356 CCATGG C^C[AT][AT]GG
HapII 501 CCGG C^CGG
HhaI 162 GCGC GCG^C
HinP1I 162 GCGC G^CGC
HpaII 501 CCGG C^CGG
HphI 413 TCACC GGTGA(8/7)
MamI 669 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 54 GATC ^GATC
MflI 53 GGATCC [GA]^GATC[CT]
MspI 501 CCGG C^CGG
MwoI 240 GCCAAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 356 CCATGG C^CATGG
NdeI 550 CATATG CA^TATG
NdeII 54 GATC ^GATC
NheI 226 GCTAGC G^CTAGC
PleI 337 GAGTC GAGTC(4/5)
Sau3AI 54 GATC ^GATC
ScaI 486 AGTACT AGT^ACT
SfcI 623 CTGTAG C^T[GA][CT]AG
SpeI 45 ACTAGT A^CTAGT
SphI 197 GCATGC GCATG^C
SspBI 182 TGTACA T^GTACA
SspI 255 AATATT AAT^ATT
StuI 60 AGGCCT AGG^CCT
StyI 356 CCATGG C^C[AT][AT]GG
XhoII 53 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 54 GATCC GGATC(4/5)
53 GGATC
AvaII 366 GGACC G^G[AT]CC
855 GGTCC
BsiYI 369 CCGTTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCAGAAGGAGG
BslI 369 CCGTTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCAGAAGGAGG
BsmAI 167 GTCTC GTCTC(1/5)
804 GTCTC
BsmI 718 GCATTC GAATGC(1/-1)
908 GCATTC
BssKI 58 CCAGG ^CC[ACGT]GG
363 CCTGG
BstNI 58 CCAGG CC^[AT]GG
363 CCTGG
EcoRII 58 CCAGG ^CC[AT]GG
363 CCTGG
HincII 39 GTTGAC GT[CT]^[GA]AC
589 GTCAAC
HindII 39 GTTGAC GT[CT]^[GA]AC
589 GTCAAC
MseI 266 TTAA T^TAA
713 TTAA
MvaI 58 CCAGG CC^[AT]GG
363 CCTGG
ScrFI 58 CCAGG CC^[ACGT]GG
363 CCTGG
Tru9I 266 TTAA T^TAA
713 TTAA
Tsp509I 130 AATT ^AATT
509 AATT
TspRI 76 CAGTG CA[GC]TG[ACGT][ACGT]^
621 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI BalI BbvI BfaI BsaJI
BsrDI BstF5I Cac8I Cfr13I DdeI
EaeI Fnu4HI FokI HaeIII HinfI
ItaI MaeI MboII MluNI MnlI
MscI MslI NlaIII NlaIV NspI
RsaI Sau96I SfaNI TfiI TseI